Summary
Alloxan induced diabetes in rats was associated with a significant reduction in the acetylcholine esterase activity of the erythrocyte membrane. Preincubation of these membranes with insulin caused a rapid but transient stimulation of this enzyme activity in both normal and diabetic rats, the effect being more marked in the latter group.
References
Acarwal V. R., Rastogi A. K., Sahib M. K., Sagar P.:In vitro insulin action on different ATPases of erythrocyte membranes in normal and diabetic rats — Acta diabetol. lat.22, 111, 1985.
Catalán R. E., Martinez A. M., Mata F., Aragonés M. D.: Effect of insulin on acetylcholinesterase activity — Biochem. Biophys. Res. Commun.101, 1216, 1981.
Dodge J. T., Mitchell C., Hanahan D. J.: The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes — Arch. Biochem. Biophys.100, 119, 1963.
Ellman G. L., Courtney K. D., Andres V. Jr., Featherstone R. M.: A new and rapid colorimetric determination of acetylcholinesterase activity — Biochem. Pharmacol.7, 88, 1961.
Gandhi C. R., Chowdhury D.: Effect of diabetes mellitus on 2-membrane integrated enzymes of erythrocytes of different ages — Indian J. exp. Biol.18, 620, 1980.
Im J. H., Cuppoletti J., Meezan E., Rackley C. E., Kim H. D.: Distribution of insulin receptors in human erythrocytes membranes. Insulin binding to sealed right side out and inside out human erythrocyte vesicles — Biochim. biophys. Acta (Amst.)775, 260, 1984.
Jeffrey P. D.: Controversial topics: the mechanism of insulin action. The interaction of insulin with its receptor. Cross-linking via insulin association as the source of receptor clustering — Diabetologia23, 381, 1982.
Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J.: Protein measurement with the Folin phenol reagent — J. biol. Chem.193, 265, 1951.
Marshall S., Olefsky J. M.: The endocytotic-internalization pathway of insulin metabolism: relationship to insulin degradation and activation of glucose transport — Endocrinology107, 1937, 1980.
Okamoto M., Kuzuya H., Kuo P., Imura H.: Insulin receptors on erythrocytes and hepatocytes from streptozotocin induced diabetic rats — Endocrinol. jap.29, 623, 1982.
Steck T. L.: Preparation of impermeable inside out and right side out vesicles from erythrocyte membranes — Meth. memb. Biol.2, 245, 1974.
Subbiah M. T. R., Yunker R. L.: Evidence for the presence of non-lipoprotein factors in diabetic serum capable of stimulating rat hepatic cholesterol-7-α-hydroxylasein vitro — Biochem. biophys. Res. Commun.121, 743, 1984.
Author information
Authors and Affiliations
Additional information
CDRI Communication No. 3721.
Rights and permissions
About this article
Cite this article
Agarwal, V.R., Rastogi, A.K., Sahib, M.K. et al. In vitro insulin effect on acetylcholine esterase of erythrocyte membranes of normal and diabetic rats. Acta diabet. lat 22, 358–363 (1985). https://doi.org/10.1007/BF02624755
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02624755