Editor's Statement This article reports a creative technical application of the author's previous work on lipid metabolism in lymphoid cells allowing an efficient, alternative selection procedure for isolation of hybridomas.
Summary
We have used the cholesterol auxotrophy of NS-1 mouse myeloma cells as the basis for selecting NS-1 hybridomas. The outgrowth of nascent NS-1 hybridomas in cholesterol-free serum-free medium was 3- to 9-fold more efficient than that in HAT medium and resulted in 3- to 13-times as many antigen-reactive hybridoma wells. This method of hybridoma selection can be applied with any sterol-dependent parent cell line. Hybridomas established under serum-free culture conditions were growth inhibited by fetal calf serum.
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This work was supported by the Japanese Ministry of Education, Science and Culture and in part by grants from the National Institutes of Health.
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Myoken, Y., Okamoto, T., Osaki, T. et al. An alternative method for the isolation of NS-1 hybridomas using cholesterol auxotrophy of NS-1 mouse myeloma cells. In Vitro Cell Dev Biol 25, 477–480 (1989). https://doi.org/10.1007/BF02624636
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DOI: https://doi.org/10.1007/BF02624636