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A simplified technique for the short-term tissue culture of rabbit corneal cells

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Summary

A technique for the short-term culture of pure populations of rabbit corneal endothelial and epithelial cells has been developed. Rabbit corneas were placed on concave agarose surfaces, treated briefly with a solution of trypsin and ethylenediamine tetracetic acid, and transferred, either epithelial cell surface or endothelial cell surface down, to microscope slide culture chambers. Within 6 to 12 h the epithelial cells or endothelial cells attached to the slide chamber surface and the cornea was removed, leaving behind a pure population of cells which spread out and grew to fill the surface of the slide chamber. This technique provides a simple and economic means for the reproducible initiation of primary cultures of rabbit corneal epithelial and endothelial cells for us in a variety of experiments.

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Authors and Affiliations

  1. Lions Eye Research Laboratories, LSU Eye Center, Louisiana State University Medical Center School of Medicine, 2020 Gravier St., Suite B., 70112, New Orleans, Louisiana

    Lixin Xie & Bryan M. Gebhardt

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  1. Lixin Xie
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  2. Bryan M. Gebhardt
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Additional information

This study was supported in part by Public Health Service grants EY03150, EY02580, and EY02377 from the National Eye Institute, National Institutes of Health, Bethesda, MD, and a Foreign Fellowship (Dr. Xie) from Research to Prevent Blindness, Inc., New York, NY.

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Xie, L., Gebhardt, B.M. A simplified technique for the short-term tissue culture of rabbit corneal cells. In Vitro Cell Dev Biol 25, 20–22 (1989). https://doi.org/10.1007/BF02624406

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  • DOI: https://doi.org/10.1007/BF02624406

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