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Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium

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Editor's Statement These results help identify the defect in myeloma cells leading to cholesterol auxotrophy. The use of these cells in hybridoma derivation adds practical utility to a detailed appreciation of cholesterol metabolism in these cultures.

Summary

The proximate cholesterol precursors lathosterol, 7-dehydrocholesterol and desmosterol supported the growth of NS-1 and X63 mouse myeloma cells. These cells and X63.653 cells are cholesterol auxotrophs, yet each was able to convert [3H]lathosterol to [3H]cholesterol. These results are consistent with the conclusion that cholesterol auxotrophy in these myeloma cells is due to a deficiency in 3-ketosteroid reductase activity. The steroid hormones testosterone, progesserone and hydrocortisone could not replace cholesterol as a medium supplement. These results provide a greater understanding of the cholesterol auxotrophy characteristic of cell lines clonally-derived from the MOPC 21 myeloma tumor, and they provide a rational basis for the use of sterols in defined culture medium for mouse myeloma cells.

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Authors and Affiliations

  1. W. Alton Jones Cell Science Center, Inc., 10 Old Barn Road, 12946, Lake Placid, New York

    J. Denry Sato, Hui-Ting Gao, Yoshiaki Kayada, Myles C. Cabot, Gordon H. Sato, Tetsuji Okamoto & Clement J. Welsh

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  1. J. Denry Sato
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  2. Hui-Ting Gao
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  3. Yoshiaki Kayada
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  4. Myles C. Cabot
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  5. Gordon H. Sato
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  6. Tetsuji Okamoto
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  7. Clement J. Welsh
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Additional information

This work was supported by National Institute of Health grants CA40294 and CA37589 to G. H. Sato and by a grant from RJR nabisco Inc.

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Sato, J.D., Gao, HT., Kayada, Y. et al. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell Dev Biol 24, 1223–1228 (1988). https://doi.org/10.1007/BF02624194

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