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A general procedure for protoplast recovery, callus and plant regeneration in plants

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References

  1. Gamborg, O. L.; Miller, R. A.; Ojima, K. Nutrient requirements of suspension culture of soybean root cells. Exp. Cell Res. 50:151–158; 1968.

    Article  PubMed  CAS  Google Scholar 

  2. Jefferson, R. A. Assaying chimeric genes in plants: The GUS gene fusion system. Pl. Mol. Biol. Rep. 5(4):387–405; 1987.

    Article  CAS  Google Scholar 

  3. Kao, K. N.; Michayluk, M. R. Nutritional requirements for growth ofVicia hajastana cells and protoplasts at a very low population density in liquid media. Planta. 126:105–110; 1975.

    Article  CAS  Google Scholar 

  4. Kyozuka, J.; Hayashi, Y.; Shimamoto, K. High frequency of plant regeneration from rice protoplasts by novel nurse culture methods. Mol. Gen. Genet. 206:408–413; 1987.

    Article  CAS  Google Scholar 

  5. Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  6. Polacco, J. C. Nitrogen metabolism in soybean tissue culture. I. Assimilation of Urea. Plant Physiol. 58:350–357; 1976.

    Article  PubMed  CAS  Google Scholar 

  7. Zaghmount, O. M. F. Isolation and culture of protoplasts from embryogenic cell suspension culture ofLolium perenne L. andFestuca rubra L. Ph.D. dissertation 1988. University of Massachusetts/Amherst. (Diss. Abst., 88–13291).

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Authors and Affiliations

  1. Department of Biochemistry 117 Schweitzer Hall, University of Missouri, 65211, Columbia, MO

    Ousama M. Faiz Zaghmout, Mark A. Holland & Joseph C. Polacco

  2. Department of Plant and Soil Science, University of Massachusetts, 01003, Amherst, MA

    William A. Torrello

Authors
  1. Ousama M. Faiz Zaghmout
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  2. William A. Torrello
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  3. Mark A. Holland
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  4. Joseph C. Polacco
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Additional information

A portion of this work was supported by NSF grant DCB 8718314 and by the Missouri Agricultural Experiment Station. This research is a joint contribution from the Missouri Agricultural Experiment Station, Journal Series No. 10,789 and from the Massachusetts Agricultural Experiment Station, Amherst, MA 01003; Journal Paper No. MAES 2959. Ousama Zaghmout was supported by the Food for the 21st Century Program, College of Agriculture, University of Missouri, Columbia, MO.

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Faiz Zaghmout, O.M., Torrello, W.A., Holland, M.A. et al. A general procedure for protoplast recovery, callus and plant regeneration in plants. In Vitro Cell Dev Biol 26, 315–317 (1990). https://doi.org/10.1007/BF02623820

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