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Modulation of β-receptors as adult and neonatal cardiac myocytes progress into culture

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Summary

Modulation of β-adrenergic receptors and their ability to respond to β-receptor stimulation was studied in cultures of adult and neonatal rat cardiac myocytes. The radioligand iodocyanopindolol (125I-CYP) was used to identify β-adrenoceptors on the intact cells.125I-CYP was found to bind to the receptors in a stereospecific and saturable manner. Freshly isolated neonatal and adult myocytes both had a receptor density of approximately 50 fmol/mg protein. The number of β-receptors per milligram protein was similar during a 10-d culture period for adult myocytes but increased after a 5-d culture period for neonatal myocytes. Both cell types responded to β-receptor stimulation with isoproterenol by a twofold increase in the concentration of cAMP and this response increased with time in culture. The number of receptors as well as the response to isoproterenol was similar for neonatal myocytes cultured on laminin, collagen type I, or on uncoated culture dishes. From these data we conclude that cultured cardiac myocytes maintain functional β-receptors as they progress into culture, and the expression of β-receptors is not influenced by culture substrates.

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Authors and Affiliations

  1. Department of Medical and Physiological Chemistry, University, Box 575, S-751 23, Uppsala, Sweden

    Evy Lundgren

  2. Department of Anatomy, University of South Carolina, 29208, Columbia, South Carolina

    Louis Terracio

  3. Department of Pharmacology, University of South Carolina, 29208, Columbia, South Carolina

    Donald O. Allen

  4. Department of Pathology, University of South Carolina, 29208, Columbia, South Carolina

    Thomas K. Borg

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  1. Evy Lundgren
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  2. Louis Terracio
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  3. Donald O. Allen
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  4. Thomas K. Borg
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Additional information

This investigation was supported by grants HL 24935 and HL 33656 from the National Institutes of Health, Bethesda, MD, and Swedish Medical Research Council grant 07466.

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Lundgren, E., Terracio, L., Allen, D.O. et al. Modulation of β-receptors as adult and neonatal cardiac myocytes progress into culture. In Vitro Cell Dev Biol 24, 28–34 (1988). https://doi.org/10.1007/BF02623812

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