Editor’s Statement This procedure for growing stationary suspension cultures in an agar slurry should be useful for shipping suspensions and for long-term maintenance of little used or back-up cultures.
Summary
Stationary culture of plant cell suspensions has been achieved. Slurries, produced when small amounts of agar (0.1–0.4%) were added to culture media, were used to suspend plant cells. Growth proceeded more slowly than in standard shake culture, but cells remained viable for months of culture. This method of growing plant cells in stationary culture should be useful for general applications including long-term cell culture, shipment of cultures, and physiological, molecular biological, and pathological studies.
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Christen, A.A., Gibson, D.M. A method for growing stationary plant suspension cell cultures. In Vitro Cell Dev Biol 23, 315–316 (1987). https://doi.org/10.1007/BF02623716
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DOI: https://doi.org/10.1007/BF02623716