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Proteoglycans synthesized by cultured fibroblasts derived from normal and inflamed human gingiva

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Summary

The in vitro proliferations rates and ptoteoglycans synthesized by adult human gingival fibroblasts derived from six age- and sex-matched donors of healthy and chronically inflamed gingiva were analyzed. Fibroblasts from inflamed gingiva demonstrated a slower growth rate than cells from healthy tissue. The rate of incorporation of [35S]sulfate into cell layer-associated proteoglycans and the release of these macromolecules into the culture medium did not differ appreciably between the two groups of cells. Similarly, no detectable differences in the overall charge of the proteoglycans synthesized by normal and inflamed gingival fibroblasts, as assessed by their elution from DEAE-Sephacel, were noted. However, sepharose CL-4B chromatography revealed that the medium-associated proteoglycans made by the inflamed tissue fibroblasts were depleted in one species of chondroitin sulfate proteoglycans and contained more dermatan sulfate than did control cells. In addition, the intracellular proteoglycan pool was found to be greatly diminished in the inflamed tissue fibroblast cell layers. Glycosaminoglycan analysis of the proteoglycans confirmed these observations. Compared to normal gingival fibroblasts, the inflamed tissue fibroblasts released less heparan sulfate into the medium. Additionally, increased levels of dermatan sulfate and depleted amounts of chondroitin sulfate in the medium of inflamed gingival cells were noted. The observed changes were stable through several transfers in culture and indicate that chronically inflamed tissue may contain fibroblasts mainfesting a heritable phenotype differing from fibroblasts in normal connective tissue.

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Author notes

  1. P. Mark Bartold

    Present address: Department of Pathology, University of Adelaide, Adelaide, South Australia, Australia

Authors and Affiliations

  1. Department of Pathology, School of Medicine, University of Washington, 98195, Seattle, Washington

    P. Mark Bartold & Roy C. Page

  2. Department of Periodontics, School of Dentistry, University of Washington, 98195, Seattle, Washington

    P. Mark Bartold & Roy C. Page

  3. Center for Research in Oral Biology, University of Washington, 98195, Seattle, Washington

    P. Mark Bartold & Roy C. Page

Authors
  1. P. Mark Bartold
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  2. Roy C. Page
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Additional information

P. Mark Bartold was supported by a C. J. Martin Fellowship for the National Health and Medical Research Council of Australia. This work was also supported by grants DE-03301 and DE-02600 from the National Institutes of Health, Bethesda, MD.

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Bartold, P.M., Page, R.C. Proteoglycans synthesized by cultured fibroblasts derived from normal and inflamed human gingiva. In Vitro Cell Dev Biol 22, 407–417 (1986). https://doi.org/10.1007/BF02623531

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  • DOI: https://doi.org/10.1007/BF02623531

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