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Cytomorphological lesions induced by chemotherapeutic agents in transitional cell carcinoma in vitro

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Summary

Transitional cell carcinoma from 20 patients and two human cell lines were maintained in short-term tissue culture. Each was studied ultrastructurally before and after incubaton with cisplatinum, adriamycin, or mitomycin C. Sequential ultrastructural changes were noted and were found to be specific for each agent tested. Ultrastructural changes in the nucleoli were produced by exposure to cisplatinum or mitomycin C; alterations in the heterochromatin of the nuclei were characteristic of treatment with adriamycin. The changes in the nucleoli seen with cisplatinum have not been described previously and support an alkylating property as a mechanism of action. Intravesical chemotherapeutic agents are now commonly used in clinical treatments. The morphological changes produced by these agents are specific and may be seen in the clinical setting.

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Authors and Affiliations

  1. Department of Surgery, Division of Urology, Harry S. Truman Memorial Veterans Hospital, 65201, Columbia, Missouri

    D. J. Weaver

  2. Medical Research Service, Harry S. Truman Memorial Veterans Hospital, 65201, Columbia, Missouri

    B. A. L. Barrett

  3. Pathology Service, Harry S. Truman Memorial Veterans Hospital, 65201, Columbia, Missouri

    E. H. Adelstein

  4. Division of Urology, University of Missouri Health Sciences Center, 65212, Columbia, Missouri

    G. Ross Jr.

Authors
  1. D. J. Weaver
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  2. B. A. L. Barrett
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  3. G. Ross Jr.
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  4. E. H. Adelstein
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Additional information

This research was supported by the Veteran's Administration Merit Review grant.

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Weaver, D.J., Barrett, B.A.L., Ross, G. et al. Cytomorphological lesions induced by chemotherapeutic agents in transitional cell carcinoma in vitro. In Vitro Cell Dev Biol 22, 653–660 (1986). https://doi.org/10.1007/BF02623479

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  • DOI: https://doi.org/10.1007/BF02623479

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