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Effects of retionoids on invasion of organ cultures of chick chorioallantoic membrane by adenovirus transformed cells

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Summary

Invasion of chick chorioallantoic membrane (CAM) organ cultures by rat 3Y1 cells transformed by the highly oncogenic human adenovirus type 12 (3Y1/12-10 cells) was inhibited by several retinoids tested. The anti-invasive activity of the retinoids was dependent on retinoid concentration and continuous (4d) exposure of the CAM. The 50% retinoid dose (dose effective in achieving a response in half of the organ cultures) that inhibited invasion was 0.85 μg/ml of retinol palmitate, 0.39 μg/ml of retinoic acid, or 0.16 μg/ml of retinol acetate. This dose was of the same order of magnitude as that which induced CAM differentiation, and was three-to fourfold less than the dose that caused cytotoxic damage of CAM. In addition, the retinoids inhibited 3Y1/12-10 cell growth by approximately 40% at levels over 10-fold higher than those needed for anti-invasion activity. The findings suggest that the anti-invasive activity of retinoids was at least partly due to direct induction of cell differentiation of the CAM host tissue.

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Authors and Affiliations

  1. Department of Biology, University of Akron, 44325, Akron, Ohio

    Fatemeh Fazely, Dorothy C. Moses & Nada Ledinko

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  1. Fatemeh Fazely
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  2. Dorothy C. Moses
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  3. Nada Ledinko
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Additional information

This work was supported by National Cancer Institute Grant CA 13231 and by University of Akron Grant RG 832.

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Fazely, F., Moses, D.C. & Ledinko, N. Effects of retionoids on invasion of organ cultures of chick chorioallantoic membrane by adenovirus transformed cells. In Vitro Cell Dev Biol 21, 409–414 (1985). https://doi.org/10.1007/BF02623472

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  • DOI: https://doi.org/10.1007/BF02623472

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