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Establishment of a continuous cell line from fibrotic schistosomal granulomas in mice livers

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Summary

A continuous murine cell line (GRX) was obtained from fibrotic granulomas induced in C3H/HeN mice liver by experimental infection withSchistosoma mansoni. This anchorage-dependent line produces composite connective tissue/extracellular matrix, displays morphological characteristics of myofibroblasts, and can, under appropriate conditions, accumulate fat droplets. GRX cells produce viral particles of retrovirus type. We consider GRX cell line to be representative of liver connective tissue cells, responsible for fibroplasia in liver fibrotic and granulomatous reactions.

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Authors and Affiliations

  1. Institut Pasteur, CNRS ERA 819, 77 Rue Pasteur, 69007, Lyon, France

    Radovan Borojevic & Hervé Emonard

  2. Departamento de Bioquimica, Instituto de Quimica, Universidade Federal do Rio de Janeiro, 21941 Ihla do Fundão, Rio de Janeiro, Brasil

    Radovan Borojevic, Alvaro N. A. Monteiro, Solange A. Vinhas, Gilberto B. Domont & Jean-Alexis Grimaud

  3. Departamento de Bioquimica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, 21941 Ilha do Fundão, Rio de Janeiro, Brasil

    Paulo A. S. Mourão

  4. Centro de Microscopia Eletrônica, Instituto Oswaldo Cruz, 21040, Rio de Janeiro, Brasil

    Gabriel Grimaldi Jr.

Authors
  1. Radovan Borojevic
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  2. Alvaro N. A. Monteiro
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  3. Solange A. Vinhas
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  4. Gilberto B. Domont
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  5. Paulo A. S. Mourão
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  6. Hervé Emonard
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  7. Gabriel Grimaldi Jr.
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  8. Jean-Alexis Grimaud
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Additional information

This research was supported by Centre National de la Recherche Scientifique, France; Financiadora de Estudos e Projetos (FINEP), Brasil; and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brasil.

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Borojevic, R., Monteiro, A.N.A., Vinhas, S.A. et al. Establishment of a continuous cell line from fibrotic schistosomal granulomas in mice livers. In Vitro Cell Dev Biol 21, 382–390 (1985). https://doi.org/10.1007/BF02623469

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  • DOI: https://doi.org/10.1007/BF02623469

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