Summary
Smooth muscle cell cultures were prepared from stomach explants obtained surgically from 10 patients with duodenal ulcer. The cultured cells grew in either overlapping layers in “hills and valleys” or in parallel arrays. The ultrastructure studies showed plasmalemmal vesicles, bundles of myofilaments associated with dense bodies, and gap junctions. The synthesis of contractile proteins illustrated the preponderance of actin on myosin and tropomyosin. The synthesis of contractile proteins in stomach smooth muscle cell cultures is significantly higher than in skin fibroblast cultures, i.e. 20 x higher for myosin, 10 x higher for actin, and 30 x higher for tropomyosin.
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Wu, C. J.; Rojkind, M.; Rifas, L.; Seifter, S. Biosynthesis of type I and type III collagens by cultured uterine smooth muscle cells. Arch. Biochem. Biophys. 188:295–300;1978.
Chamley, J. H.; Campbell, G. R.; McConnell, J. D. Comparison of vascular smooth muscle cells from adult human, monkey and rabbit in primary culture and in subculture. Cell Tissue Res. 177:503–522; 1977.
Grosche-Stewart, U.; Chamley, J. H.; McConnell, J. D.; Burnstock, G. Comparison of the reaction of cultured smooth and cardiac muscle cells and fibroblasts to specific antibodies to myosin. Histochemistry 43:215–224;1975.
Fowler, S.; Shio, H.; Wolinsky, H. Subcellular fractionation and morphology of calf aortic smooth muscle cells. J. Cell Biol. 75:166–184;1977.
Campbell, G. R.; Uehara, Y.; Mark, G.; Burnstock, G. Fine structure of smooth muscle cells grown in tissue culture. J. Cell Biol. 49:21–34; 1971.
Haudenschild, C. C.; Cotran, R. S.; Gimbrione, M. A., Jr.; Folkman, J. Fine structure of vascular endothelium in culture. J. Ultrastruct. Res. 50:22–32; 1975.
Ingwall, J. S.; Morales, M. F.; Stockdale, F. E. Creatine and the control of myosin synthesis in differentiating skeletal muscle. Proc. Natl. Acad. Sci. USA 69:2250–2253; 1972.
Whalen, R. G.; Butler-Brown, G. S.; Gros, F. Protein synthesis and actin heterogeneity in calf muscle cells in culture. Proc. Natl. Acad. Sci. USA 73:2013–2022; 1972.
Allen, R.; Stromer, M.; Goll, D. Synthesis of tropomyosin in cultures of differentiating muscle cells. J. Cell Biol. 76:98–104; 1978.
Weber, K.; Osborn, M. The reliability of molecular weight determinations by dodecylsulfate polyacrylamide gel electrophoresis. J. Biol. Chem. 244:4406–4412; 1969.
Starr, R.; Offer, G. Polypeptide chains of intermediate molecular weight in myosin preparations. FEBS Lett. 15:40–44; 1971.
Lowry, O. H.; Rosebrough, N. J.; Farr, A. L. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 254–275; 1951.
Stahl, E. Thin layer chromatography. Berlin: Springer-Verlag; 1969.
Ross, R. The smooth muscle cell. II. Growth of smooth muscle in culture and formation of elastic fibers. J. Cell Biol. 50:172–186; 1971.
Chamley-Campbell, J.; Campbell, G. R.; Ross, R. Physiological reviews: The smooth muscle cell in culture. Am. J. Physiol. 59: 1–61; 1979.
Garfield, R. E. Regeneration of smooth muscle: ultrastructure and multipotential properties of smooth muscle. In: Mauro, A.; Shafiq, S.; Milhorat, A., eds. Muscle regeneration. New York: Raven Press; 1979.
Campbell, G. R.; Chamley, J. H.; Burnstock, G. Development of smooth muscle cells in tissue cultures. J. Anat. 117:295–312; 1974.
Devine, C. E.; Somlyo, A. P. Thick filaments in vascular smooth muscle. J. Cell Biol. 49:638–649; 1971.
Bois, R. M. The organization of the contractile apparatus of vertebrate smooth muscle. Anat. Rec. 177:61–78; 1973.
Somlyo, A. P.; Devine, C. E.; Somlyo, A. V.; Rice, R. V. Filament organization in vertebrate smooth muscle. Philos. Trans. R. Soc. Lond. [Biol.] 265:223–229; 1973.
Young, R. B.; Bergen, W. G.; Blauwickel, P. B. Myosin synthesis in embryonic chick fibroblasts. J. Cell Biol. 81: 115–122; 1979.
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Ionasescu, R., Ionasescu, V. Human smooth muscle cell cultures of the stomach morphologic and biochemical studies. In Vitro Cell Dev Biol 21, 368–372 (1985). https://doi.org/10.1007/BF02623467
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DOI: https://doi.org/10.1007/BF02623467