Summary
We report that synchronized cultures ofPlasmodium falciparum with up to 40% parasitized cells can be obtained with the use of low, red blood cell suspensions and only daily replacement of culture medium. These cultures contained not only a reduced proportion of uninfected red cells but also of population of cells with brief and equal time of exposure to culture conditions. Such high parasitemias are desirable for studies of ring-staged parasites (for which enrichment techniques are not available) and late-staged parasites when the manipulations for enrichment are inappropriate or unsuccessful.
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This work was supported by Grant HL-21016 from the National Institutes of Health, Bethesda, MD
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Raventos-Suarez, C. Plasmodium falciparum: Invasion and development in highly parasitized cultures. In Vitro Cell Dev Biol 21, 161–164 (1985). https://doi.org/10.1007/BF02621353
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DOI: https://doi.org/10.1007/BF02621353