Skip to main content

Advertisement

SpringerLink
Log in

Immuno-isolation and culture of biliary epithelial cells from normal human liver

  • Rapid Communications in Cell Biology
  • Published:
In Vitro Cellular & Developmental Biology Aims and scope Submit manuscript

Summary

Biliary epithelial cells (BEC) lining the intra-hepatic biliary ducts are the site of damage in several immunologically mediated liver diseases. BEC are difficult to isolate since they represent only 5% of the total cell number in normal liver. In this communication, a novel method for their isolation from normal liver is presented using a monoclonal antibody (HEA125) with specificity for an epithelial cell surface glyco-protein reported to be expressed in liver only by biliary epithelium. By combining differential density centrifugation and immuno-magnetic separation using HEA125 pure BEC (105 cells/g fresh tissue) were prepared routinely. These cells were maintained in culture for up to 4 weeks with significant increases in cell numbers. The ability to prepare BEC from human liver offers an opportunity to develop In Vitro models to investigate the aetiology of diseases in intra-hepatic biliary epithelium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Allen-Hoffman, B. L.; Rheinwald, J. G. Polycyclic aromatic hydrocarbon mutagenesis of human epidermal keratinocytes in culture. Proc. Natl. Acad. Sci. USA 81:7802–7806; 1984.

    Article  Google Scholar 

  2. Daoust, R. In: Brauer, R. E., ed. Liver Function. Publication 4. American Institute Biological Sciences: Waverly Press; 1958:3.

  3. de Hemptinne, B.; Salizzoni, M.; Yandza, T. C., et al. Indication, technique and results of liver graft volume reduction before orthotopic transplantation in children. Transplantation Proceedings 19(5):3549–3551; 1987.

    PubMed  Google Scholar 

  4. Demetris, A. J.; Markus, B. H.; Saidman, S., et al. Isolation and primary cultures of human intrahepatic bile ductular epithelium. In Vitro Cell. Dev. Biol. 24(5):464–470; 1988.

    PubMed  CAS  Google Scholar 

  5. Gall, J. A. M.; Bathal, P. S. The isolation of intrahepatic biliary epithelial cells from normal rat livers. Cell. Biol. Int. Rep. 9:315–322; 1985.

    Article  PubMed  CAS  Google Scholar 

  6. Grant, A. C.; Billing, B. H. The isolation and characterization of a bile ductule cell population from normal and bile duct ligated rat livers. Br. J. Exp. Pathol. 58:301–310; 1977.

    PubMed  CAS  Google Scholar 

  7. Kalayoglu, M.; Sollinger, H. W.; Stratta, R. J., et al. Extended preservation of liver for clinical transplantation. Lancet 1:617–619; 1988.

    PubMed  CAS  Google Scholar 

  8. Kirby, R. M.; McMaster, P.; Clements, D., et al. Orthotopic liver transplantation: post operative complications and their management. Br. J. Surg. 74:3–11; 1987.

    Article  PubMed  CAS  Google Scholar 

  9. Leivestad, T.; Gaudernack, G.; Ugelstad, J., et al. Isolation of pure and functionally active T4 and T8 cells by positive selection with antibody-coated monosized magnetic microspheres. Transplantation Proceedings 18(1):265–267; 1987.

    Google Scholar 

  10. Momburg, F.; Moldenhauer, G.; Hammerling, G. H., et al. Immunohistochemical study of the surface expression of a M4 34,000 human epithelium-specific glycoprotein in normal and malignant tissues Cancer Res. 47:2883–2891; 1987.

    PubMed  CAS  Google Scholar 

  11. Parola, M.; Cheeseman, K. H.; Biocca, M. E. et al. Isolation and characterization of biliary epithelial cells from noraml rat liver. J. Hepatol. 6: 175–186; 1988.

    Article  PubMed  CAS  Google Scholar 

  12. Rheinwald, J. G.; Green, H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 6:331–343; 1975

    Article  PubMed  CAS  Google Scholar 

  13. Rutenberg, A. M.; Kim, H.; Fischbein, J. W., et al. Histochemical and ultrastructural demonstration of gammaglutamyl transpeptidase activity. J. Histochem. Cytochem. 17:517–526; 1969.

    Google Scholar 

  14. Schaffner, F.; Popper, H. In: Popper, H.; Schaffner, F., eds. Progress in liver disease Vol. 7. Clinical-pathologic relations in Primary Biliary Cirrhosis. New York: Grune and Stratton: 1982:529–554.

    Google Scholar 

  15. Stanley, M. A.; Parkinson, E. K. Growth requirements of human cervical cells in culture. Int. J. Cancer 24:407–414; 1979.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medicine, University of Birmingham/Liver Unit, Queen Elizabeth Hospital, B15 2TH, Birmingham, UK

    R. Joplin & J. M. Neuberger

  2. Department of Biochemistry, University of Birmingham/Liver Unit, Queen Elizabeth Hospital, Birmingham, B15 2TH, Birmingham, UK

    A. J. Strain

Authors
  1. R. Joplin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. J. Strain
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. M. Neuberger
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

EDITOR’S STATEMENT This is a novel application to purification of specific liver cell types directly from tissue. It is well-suited for rapid communication because of its novelty and potential utility to investigators.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Joplin, R., Strain, A.J. & Neuberger, J.M. Immuno-isolation and culture of biliary epithelial cells from normal human liver. In Vitro Cell Dev Biol 25, 1189–1192 (1989). https://doi.org/10.1007/BF02621273

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02621273

Key words

Search

Navigation