Summary
Attempts were made to select for trophoblast cells in cultures of mixed cell populations derived from preterm (7 to 12 wk) or term human placentas. Epidermal growth factor added to cultures on solid or porous supports caused proliferation of epithelial-type cells to give a confluent monolayer but did not increase the expression of differentiated function. The presence or absence of placental basement membrane collagen as substrate made little apparent difference; however a porous basement membrane collagen support led to increased differentiated function. Initial production of human chorionic gonadotrophin was increased and after 4 wk in culture a substantial proportion of the cells exhibited alkaline phosphatase activity. Epidermal growth factor and a substrate of placental basement membrane collagen on a porous support favorably influence the growth and differentiation of human trophoblast cells in culture.
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References
Aladjem, S.; Lueck, J. Morphologic characteristics of the normal term human trophoblast maintained in prolongedin vitro cultures. Br. J. Obstet. Gynaecol. 88: 287–293; 1981.
Bancroft, J. D.; Stevens, A. Theory and praetice of histological techniques, 2nd ed. Edinburgh: Churchill Livingstone; 1982.
Bottenstein, J.; Hayashi, I.; Hutchings, S., et al. The growth of cells in serum-free hormone-supplemented media. Methods Enzymol. 58: 94–109; 1979.
Boyd, J. D.; Hamilton, W. J. The human placenta. Cambridge: Heffer and Sons Ltd.; 1970: 140–174.
Cereijido, M.; Robbins, E. S.; Dolan, W. J., et al. Polarized monolayers formed by epithelial cells on a permeable and translucent support. J. Cell Biol. 77: 853–880; 1978.
Cotte, C.; Easty, G. C.; Neville, A. M., et al. Preparation of highly purified cytotrophoblast from human placenta with subsequent modulation to form syncytiotrophoblast in monolayer cultures. In Vitro 16: 639–646; 1980.
Kleinman, H. K.; McGoodwin, E. B.; Rennard, S. I., et al. Preparation of collagen substrates for cell attachment: effect of collagen concentration and phosphate buffer. Anal. Biochem. 94: 308–312; 1979.
Loke, Y. W. Human trophoblast in culture. In: Loke, Y. W.; Whyte, A., eds. Biology of trophoblast. Amsterdam: Elsevier Science Publishers; 1983: 663–701.
Lobo, J. O.; Bellino, F. L.; Bankert, L. Estrogen synthetase activity in human term placental cells in monolayer culture. Endocrinology 116: 889–895; 1985.
McKeehan, W. L.; McKeehan, K. A.; Hammond, S. L., et al. Improved medium for clonal growth of human diploid cells at low concentrations of serum proteins. In Vitro 13: 399–416; 1977.
Morgan, D. M. L.; Toothill, V. J.; Landon, M. J. Long-term culture of human trophoblast cells. Brit. J. Obstet. Gynaecol. 92: 84–92; 1985.
Morrish, D. W.; Siy, O. Preservation of human chorionic gonadotropin and placental lactogen secretion and normal morphology following long-term culture of normal human placental cells. Life Sci. 36: 1175–1181; 1985.
Paul, S.; Jailkhani, B. L.; Talwar, G. P. Isolation and functional maintenance in culture of syncytiotrophoblasts from human placenta. Indian J. Exp. Biol. 16: 1226–1232; 1978.
Truman, P.; Ford, H. C. The brush border of the human term placenta. Biochim. Biophys. Acta 779: 139–160; 1984.
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This work was supported by funds from the Medical Research Council of New Zealand which also provided support for Dr. Truman as a Postdoctoral Fellow.
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Truman, P., Ford, H.C. The effect of substrate and epidermal growth factor on human placental trophoblast cells in culture. In Vitro Cell Dev Biol 22, 525–528 (1986). https://doi.org/10.1007/BF02621138
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DOI: https://doi.org/10.1007/BF02621138