Editor's Statement Identification of monoclonal antibodies that recognize human lung adenocarcinoma cells with reasonable specificity represents a potentially important development that may prove useful in diagnosis and therapy of neoplastic disease. The selection procedures and methods for propagation of the human-human hybridomas described in this paper also represent some novel approaches that may be of general application. David W. Barnes
Summary
Human Namalwa cells were screened in serum-free medium and in 6-thioguanine, then fused with human lymphocytes from lymph nodes of lung adenocarcinoma cancer patients. Extensive testing using 14 lung cancer cell lines, 11 other cancer cell lines and 4 normal fibroblast lines identified monoclonal antibodies produced by 4 hybridoma clones that reacted specifically with lung adenocarcinoma cells. These monoclonal antibodies also reacted with lung adenocarcinoma tissues and not normal tissues or erythrocytes of any blood type. These hybridoma clones grew and stably secreted the antibodies in serum-free medium as well as in serum-containing medium.
This is a preview of subscription content, log in via an institution to check access.
References
Colcher, D.; Horan Hand, P.; Nuti, M.; Schlom, J. A spectrum of monoclonal antibodies reactive with human mammary tumour cells. Proc. Natl. Acad. Sci. USA 78:3199–3203; 1981.
Cote, R. J.; Morrissey, D. M.; Houghton, A. N.; Beattie, E. J., Jr.; Oettgen, H. F.; Old, L. J. Generation of human monoclonal antibodies reactive with cellular antigens. Proc. Natl. Acad. Sci. USA 80:2026–2030; 1983.
Croce, C. A.; Linnenbach, A.; Hall, W.; Steplewski, Z.; Koprowski, H. Production of human hybridomas secreting antibody to measles virus. Nature 288:488–489; 1980.
Cuttitta F.; Rosen, S.; Gazdar, A. F.; Minna, J. D. Monoclonal antibodies that demonstrate specificity for several types of human lung cancer. Proc. Natl. Acad. Sci. USA 78:4591–4595; 1981.
Dorreen, M. S.; Habeshaw, J. A.; Wrigley, P. F. M.; Lister, T. A. Distribution of T-lymphocyte subsets in Hodgkin's disease characterized by monoclonal antibodies. Br. J. Cancer 45:491–497; 1982.
Glassy, M. C.; Handley, H. H.; Hagiwara, H.; Royston, I. UC 729-6, a human lymphoblastoid B-cell line useful for generating antibody-secreting human-human hybridomas. Proc. Natl. Acad. Sci. USA 80:6327–6331; 1983.
Hakomori, S.; Nudelman, E.; Levery, S. B.; Patterson, C. M. Human cancer-associated gangliosides defined by a monoclonal antibody (IB9) directed to sialosyl α2→6 galactosyl residue: a preliminary note. Biochem. Biophys. Res. Commun. 113:791–798; 1983.
Imai, K.; Ng, A. K.; Ferrone, S. Characterization of monoclonal antibodies to human melanoma-associated antigens. JNCI 66:489–496; 1981.
Kennett, R. H.; Gilbert, F. Hybrid myelomas producing antibodies against a human neuroblastoma antigen present on fetal brain. Science 203:1120–1121; 1979.
Köhler, G.; Milstein, C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495–497; 1975.
Larrick, J. W.; Truitt, K. E.; Raubitschek, A. A.; Senyk, G.; Wang, J. C. N. Characterization of human hybridomas secreting antibody to tetanus toxoid. Proc. Natl. Acad. Sci. USA 80:6376–6380; 1983.
Mitchell, K. F.; Fuhrer, J. P.; Steplewski, Z.; Koprowski, H. Biochemical characterization of human melanoma cell surfaces: dissection with monoclonal antibodies. Proc. Natl. Acad. Sci. USA 77:7287–7291; 1980.
Murakami, H.; Masui, H.; Sato, G. H.; Sueoka, N.; Chow, T. P.; Kano-Sueoka, T. Growth of hybridoma cells in serum-free medium: ethanolamine is an essential component. Proc. Natl. Acad. Sci. USA 79:1158–1162; 1982.
O'Hare, M. J.; Smith, C. M.; Edwards, P. A. W. A new human hybridoma system (LICR-LON-HMy2) and its use in the production of human monoclonal antibodies. Peeters, H. ed. Protides of Biological Fluids, Colloquium 30. Oxford: Pergamon Press; 1982:265–268.
Olsson, L.; Kaplan, H. S. Human-human hybridomas producing monoclonal antibodies of predefined specificity. Proc. Natl. Acad. Sci. USA 77:5427–5431; 1980.
Ritz, J.; Pesando, J. M.; Notis-McConarty, J.; Lazarus, H.; Schlossman, S. F. A monoclonal antibody to human acute lymphoblastic leukemia antigen. Nature 283:583–585; 1980.
Ritz, J.; Pesando, J. M.; Notis-McConarty, J.; Schlossman, S. F. Modulation of human acute lymphoblastic leukemia antigen induced by monoclonal antibodyin vitro. J. Immunol. 125:1506–1514; 1980.
Sikora, K.; Alderson, T.; Ellis, J.; Phillips, J.; Watson, J. Human hybridomas from patients with malignant disease. Br. J. Cancer 47:135–145; 1983.
Soule, H. R.; Linder, E.; Edgington, T. S. Membrane 126-kilodalton phosphoglycoprotein associated with human carcinomas identified by a hybridoma antibody to mammary carcinoma cells. Proc. Natl. Acad. Sci. USA 80:1332–1336; 1983.
Woodbury, R. G.; Brown, J. P.; Yeh, M. Y.; Hellstrom, I.; Hellstrom, K. E. Identification of a cell surface protein, p97, in human melanomas and certain other neoplasms. Proc. Natl. Acad. Sci. USA 77:2183–2187; 1980.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Murakami, H., Hashizume, S., Ohashi, H. et al. Human-human hybridomas secreting antibodies specific to human lung carcinoma. In Vitro Cell Dev Biol 21, 593–596 (1985). https://doi.org/10.1007/BF02620891
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02620891