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Differentiation-arrested rat fetal lung in primary monolayer cell culture

IV. Paradoxical effect of a “fetal” pO2 on precursor incorporation into phospholipids and hormone responsiveness

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Summary

Differentiation-arrested lung cell cultures were developed from fetal rats of various gestational ages. In contrast to previously published observations with cultures in a pO2 of ∼142 mm Hg, cultures developed in a pO2 of ∼30 mm Hg, close to the normal fetal arterial pO2, have improved plating efficiency and a slightly increased growth rate. They did not, however, show gestation-dependent increases of choline incorporation into phospholipids, nor did immature lung cell cultures respond to dexamethasone or triiodothyronine, singly or in combination, by increased choline incorporation into saturated lecithin. The incorporation of choline and glycerol into lipids suggested a mature rate of lipid synthesis by immature cultures at a pO2 ∼30 mm Hg, despite preservation of an immature morphology. Electron microscope observations revealed no gross differences between immature cultures developed at either pO2. The cellular mechanisms underlying these differences are unclear but suggest that oxygen tension may significantly influence results obtained with in vitro studies of lipid synthesis by immature lung.

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Authors and Affiliations

  1. The Department of Pediatrics, University of Western Ontario, London, Ontario, Canada

    A. K. Tanswell

  2. The Department of Obstetrics and Gynecology, University of Western Ontario, London, Ontario, Canada

    F. Possmayer & P. Harding

  3. The Department of Biochemistry, University of Western Ontario, London, Ontario, Canada

    F. Possmayer

  4. the Department of Anatomy, Queen's University, Kingston, Ontario, Canada

    M. G. Joneja

Authors
  1. A. K. Tanswell
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  2. M. G. Joneja
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  3. F. Possmayer
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  4. P. Harding
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Additional information

This work was supported by grants from the Medical Research Council of Canada, the Ontario Thoracic Society, and the Physicians' Services Incorporated Foundation.

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Tanswell, A.K., Joneja, M.G., Possmayer, F. et al. Differentiation-arrested rat fetal lung in primary monolayer cell culture. In Vitro 20, 635–641 (1984). https://doi.org/10.1007/BF02619613

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  • DOI: https://doi.org/10.1007/BF02619613

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