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8-Azaguanine resistant african green monkey kidney cell mutans (Vero 153): isolation and characterization

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Summary

A clone of Vero cells resistant to up 20 μg/ml 8-azaguanine was isolated. This clone (designated Vero 153) has a doubling rate of approximately 24 h and a maximum cell density of 10,000/mm2. Deficiency of the enzyme hypoxanthine guanine phosphoribosyl transferase (HGPRT) in Vero 153 was demonstrated by methods of radiochromatography. Vero 153 is susceptibletto hypoxanthine-thymidine-aminopterin (HAT) medium and its resistance to 8-azaguanine seems to be nonreversible. Like parenal cells, Vero 153 was also incapable of interferon production when challenged with Newcastle disease virus (NDV) or poly(inosinic acid) poly(cytidylic acid) (poly I:C). Similar chromasome complements (majority range 56 to 57) and band patterns were observed in cells harvested at Passages 10, 20, and 50. The potential use of Vero 153 for somatic cell hybridization for purposes of gene mapping, virus rescue, and the control of inteferon production is discussed.

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Authors and Affiliations

  1. Department of Microbiology, Dalhousie University, B3H 4H7, Halifax, Nova Sotia, Canada

    C. L. Y. Lee, S. H. S. Lee & K. R. Rozee

  2. Department of Pediatrics, University of Washington Medical School, Seatle, Washington

    S. H. Chen

Authors
  1. C. L. Y. Lee
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  2. S. H. S. Lee
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  3. K. R. Rozee
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  4. S. H. Chen
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Additional information

This project was supported by grant from the Medical Research Council, Canada (MT-1615).

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Lee, C.L.Y., Lee, S.H.S., Rozee, K.R. et al. 8-Azaguanine resistant african green monkey kidney cell mutans (Vero 153): isolation and characterization. In Vitro 19, 416–420 (1983). https://doi.org/10.1007/BF02619558

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  • DOI: https://doi.org/10.1007/BF02619558

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