Summary
When a suspension containing a mixture of viable and nonviable cells is layered over a dense ficoll-metrizoate solution and centrifuged, most of the viable cells are retained at the interface above the dense solution; whereas most of the nonviable cells are distributed in other fractions. The cells revovered for the interface are capable of subsequent growth in culture.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Clynes, M. M. Effect of culture conditions on the survival of mammalian cells without medium change. Biochem. Soc. Trans. 7: 30–32; 1979.
Fogh, J. ed. Human tumor cells in vitro. New York: Plenum Press; 1975.
Klein, G.; Dombos, L.; Gothoskar, B. Sensitivity of Epstein-Barr Virus (EBV) producer and nonproducer human lymphoblastoid cell lines to superinfection with EB-virus. Int. J. Cancer 10: 44–57; 1972.
Moorhead, P. S. Human tumor cell line with a quasidiploid karyotype. Exp. Cell Res. 39: 190–196; 1965.
Rasheed, S.; Nelson-Rees, W. A.; Toth, E. M., Arnstein, P.; Gardner, M. B. Characterization of a newly derived human sarcoma line (HT-1080). Cancer 33: 1027–1033; 1974.
Littlefield, J. W. Three degrees of guanylic acidinosinic acid pyrophosphorylase deficiency in mouse fibroblasts. Nature 203: 1142–1144; 1964
Thompson, E. B.; Tomkins, G. M.; Curran, J. F. Induction of tyrosine α-ketoglutarate transaminase by steroid hormones in a newly established tissue culture cell line. Proc. Natl. Acad. Sci. U.S.A. 56: 296–303; 1966.
Boyum, A. Separation of leucocytes from blood and bone marrow. Scand. J. Clin. Lab. Invest. 21: Suppl. 97; 1968.
Harris, R.; Ukaejiofo, E. O. Tissue typing using a routine one-step lymphocyte separation technique. Brit. J. Haematol. 18: 229–235; 1970.
Descriptive literature on ficoll and ficoll-paque. Pharmacia Ltd. Prince Regent Rd., Hounslow, Middlesex TW3 INE, England.
Descriptive literature on metrizoates lymphoprep. Nyegaard & Co., Oslo, Norway.
Philips, H. J. Dye exclusion tests for cell viability. Kruse, P. F.; Patterson, M. K. eds. Tissue culture methods and applications. New York: Academic Press; 1973: 406–408.
Anderson, R. E.; Sandefer, J. C.; Scalette, J. V. The phospholipid and glycoprotein composition at T and B cells. Lab. Invest. 37: 329–338; 1977.
Ford W. L.; Hunt, S. V. The preparation and labelling of lymphocytes. Weir, D. M. ed. Handbook of experimental immunology. 2nd ed. vol. 2. Oxford: Blackwell Scientific Publishers; 1973; 23.1–23.27.
Julius, M. H.; Simpson, E.; Herzenberg, L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur. J. Immunol. 3: 645–649; 1973.
Wepsic, H. T. Separation of viable tumor cells from non-viable tumor cells by floatation on bovine serum albumin. J. Natl. Cancer Inst. 45: 1031–1033; 1970.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Clynes, M.M., Smyth, H., O’Kennedy, R. et al. Separation of viable from nonviable cells using a discontinuous density gradient. In Vitro 16, 809–812 (1980). https://doi.org/10.1007/BF02619316
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02619316