Abstract
This study aimed at evaluating by a quantitative assay the fibrin clot retractile activity (FCR) of C3H embryo fibroblasts during their growth and aging in culture. Cell from primary and subsequent subcultures were tested at defined times from seeding, in a specially devised micromethod. Results indicate that cell-induced FCR has a kinetic similar to platelet-induced FCR; it depends on the number of cells and time of incubation in the system. It is absent or low in cells harvested from primary culture, then increases and remains high in the following doublings decreasing sharply at the end of the replicative life span in culture.
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This work was performed within the frame of the EORTC Cell Surface Project, Subgroup “Cell-fibrin interactions”; it was partially supported by an International Project of the Italian CNR and French CNRS and a grant on Aging from the Ministero della Sanità, Roma, Italy. Thrombin preparations used in this study were a kind gift from Dr. P. Priore (Roche, Milano, Italy). Judith Baggott, Vincenzo de Ceglie, Graziella Scalvini, and Anna Mancini helped to prepare this manuscript.
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Curatolo, L., Balconi, G., Borgia, R. et al. Fibrin clot retractile activity of mouse fibroblasts during growth and aging. In Vitro 16, 731–737 (1980). https://doi.org/10.1007/BF02619306
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DOI: https://doi.org/10.1007/BF02619306