Skip to main content
Log in

Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium

  • Published:
In Vitro Aims and scope Submit manuscript

Summary

An established insect cell line (AC20) from the leafhopperAgallia constricta has been adapted to a mammalian cell culture medium based on the formulation of two commercially available media. The cell population doubling time of the adapted line in this medium is approximately 45 hr at 30°C.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Schneider, I. 1967. Insect tissue culture. In: F. H. Wilt and N. K. Wessells (Eds.),Methods in Developmental Biology. Crowell Co., New York, pp. 543–554.

    Google Scholar 

  2. Vago, C. 1967. Invertebrate tissue culture. In: K. Maramorosch and H. Koprowski (Eds.).Methods in Virology, Vol. I. Academic Press, New York, pp. 567–602.

    Google Scholar 

  3. Brooks, M. A., and T. J. Kurtti. 1971. Insect cell and tissue culture. Annu. Rev. Entomol. 16: 27–52.

    Article  PubMed  CAS  Google Scholar 

  4. Hirumi, H., and K. Maramorosch. 1971. Cell culture of Hemiptera. In: C. Vago (Ed.).Invertebrate Tissue Culture, Vol. I. Academic Press, New York, pp. 307–339.

    Google Scholar 

  5. Marks, E. P. 1970. The action of hormones in insect cell and organ cultures. Gen. Comp. Endocrinol. 15: 280–302.

    Article  Google Scholar 

  6. Vaughn, L. 1971. Cell culture media and methods. In: C. Vago (Ed.),Invertebrate Tissue Culture, Vol. I. Academic Press, New York, pp. 3–40.

    Google Scholar 

  7. Kimura, I., and L. M. Black. 1971. Some factors affecting infectivity assays of wound tumor virus on cell monolayers from an insect vector. Virology 46: 266–276.

    Article  PubMed  CAS  Google Scholar 

  8. Hink, W. F. 1970. Established insect cell line from the cabbage looper,Trichoplusia ni. Nature (London) 226: 226–467.

    Article  Google Scholar 

  9. Hink, W. F., and B. J. Ellis. 1971. Establishment and characterization of two new cell lines (CP-1268 and CP-169) from the codling moth,Carpocapsa pomonella (with a review of culture of cells and tissues from Lepidoptera). Curr. Top. Microbiol. Immunol. 55: 19–28.

    PubMed  CAS  Google Scholar 

  10. Hirumi, H., and K. Maramorosch. 1964. Thein vitro cultivation of embryonic leafhopper tissues. Exp. Cell Res. 36: 625–631.

    Article  PubMed  CAS  Google Scholar 

  11. Hirumi, H. 1972. Morphological characterization of the leafhopper AC20 line cells. Proc. 3rd Internat. Colloq. Invertebrate Tissue Culture, Smolenice, Czechoslovakia, 1971, in press.

  12. Morgan, J. F., H. J. Morton, and R. C. Parker. 1950. Nutrition of animal cells in tissue culture. I. Initial studies on a synthetic medium. Proc. Soc. Exp. Biol. Med. 73: 1–8.

    PubMed  CAS  Google Scholar 

  13. Melnick, J. L. 1955. Tissue culture techniques and their application to original isolation, growth, and assay of poliomyelitis and orphan viruses. Ann. N. Y. Acad. Sci. 61: 754–772.

    Article  PubMed  CAS  Google Scholar 

  14. Merchant, D. L., R. H. Kahn, and W. H. Murphy. 1964.Handbook of Cell and Organ Culture, 2nd ed. Burgess Publishing Co., Minneapolis.

    Google Scholar 

  15. Hayflick, L. 1965. Tissue cultures and mycoplasmas. Tex. Rep. Biol. Med. 23: 285–303.

    PubMed  Google Scholar 

  16. Sohi, S. S., and Y. Hayashi. 1971. Metabolism ofAedes aegypti cells grown in vitro. II. Determination of cell viability. In Vitro 7: 146–151.

    Article  PubMed  CAS  Google Scholar 

  17. Yunker, C. E., and J. Cory. 1969. Colorado tick fever virus: growth in a mosquito cell line. J. Virol. 3: 631–632.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

This research was supported in part by National Science Foundation Grant GB29277X.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mc Intosh, A.H., Maramorosch, K. & Rechtoris, C. Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium. In Vitro 8, 375–378 (1973). https://doi.org/10.1007/BF02619063

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02619063

Key words

Navigation