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Organ culture of adult mouse intestine

III. Behavior of the proteins, DNA content and brush border membrane enzymatic activities

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Summary

The proteins the DNA content and the brush border membrane enzyme activities of adult mouse intestinal explants have been measured during a 24-hr organ culture. These activities were not modified in comparison with the controls at the beginning of the culture. The activity of the enzymes secreted in the medium during the 24-hr culture was equal to the activity present in the explants at the start of the culture. These results show that several metabolic functions of the intestine are fully preserved in organ culture.

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Authors and Affiliations

  1. Intestinal Biology Unit, Department of Anatomy and Cell Biology, Medical Center, University of Sherbrooke, J1H 5N4, Sherbrooke, P.Q., Canada

    A. Berteloot (Recipient of a studentship from the Medical Research Council of Canada), J. -G. Chabot (Recipient of a studentship from the Medical Research Council of Canada), D. Menard (“Chercheur-boursier” from CRSQ) & J. S. Hugon

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  1. A. Berteloot
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  2. J. -G. Chabot
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  3. D. Menard
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  4. J. S. Hugon
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This work was supported by a research grant from the Medical Research Council of Canada (to J.S.H.).

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Berteloot, A., Chabot, J.G., Menard, D. et al. Organ culture of adult mouse intestine. In Vitro 15, 294–299 (1979). https://doi.org/10.1007/BF02618954

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