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Organ culture of adult mouse intestine

II. Mitotic activity, DNA synthesis and cellular migration after 24 and 48 hours of culture

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DNA synthesis, mitotic activity and cell migration have been measured in organ culture of adult mouse jejunum during the first 48 hr. Explants cultured in DMEM-HEPES-NCTC-135 medium present a sharp decrease of mitotic activity in the first hours of culture, but mitoses are restored to 80% of the controls between 24 and 48 hr. DNA synthesis follows the same pattern. Cell migration continues during culture.

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Authors and Affiliations

  1. Intestinal Biology Unit, Department of Anatomy and Cell Biology, Medical Center, University of Sherbrooke, J1H 5N4, Sherbrooke, P.Q., Canada

    S. Ferland (Recipient of a studentship from the Medical Research Council of Canada) & J. S. Hugon

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  1. S. Ferland
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  2. J. S. Hugon
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Additional information

This work was supported by a research grant from the Medical Research Council of Canada (to J. S. H.) and partly by the F. C. A. C. Québec (to J. S. H.).

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Ferland, S., Hugon, J.S. Organ culture of adult mouse intestine. In Vitro 15, 288–293 (1979). https://doi.org/10.1007/BF02618953

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