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Characteristics of human chondrocyte cultures in completely defined medium

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Summary

Chondrocytes derived from normal human adult articular cartilage were established and maintained for over 5 months in a completely defined medium without the addition of serum or any other growth factors. At the end of 5 months, these cells were still metabolically active. The cells incorporated [3H]thymidine into DNA, incorporated [35S]sulfate into proteoglycans, and exhibited lysosomal enzyme activities. The35S-labeled proteoglycans isolated from the culture medium had elution profiles on high pressure liquid chromatography (HPCL) similar to those observed from proteoglycans from other mammalian sources. This self-contained growth competence may reflect a need produced by the unusual avascular and alymphatic character of articular cartilage.

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Authors and Affiliations

  1. Department of Orthopedic Surgery, Tufts University School of Medicine, 136 Harrison Avenue, Boston, Massachusetts 02111

    Edith R. Schwartz & Geetha Sugumaran

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  1. Edith R. Schwartz
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  2. Geetha Sugumaran
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This research was supported, in part, by Grant AM22057 from the National Institutes of Health, Bethesda, MD.

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Schwartz, E.R., Sugumaran, G. Characteristics of human chondrocyte cultures in completely defined medium. In Vitro 18, 254–260 (1982). https://doi.org/10.1007/BF02618579

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  • DOI: https://doi.org/10.1007/BF02618579

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