Summary
Cell lines derived from a murine neuroblastoma, Clone N18, were cured of theirM. hyorhinis infection by in vivo passage. The major variable determining success of this method was found to be the incubation time in vivo. Infected cells maintained in vivo for 27 d or more and then placed in culture were free of mycoplasma whereas those maintained in vivo for 7 or 14 d were found to still be infected. This approach to eliminating mycoplasma infection may be successful using other tumor cell lines.
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References
Robinson, L. B.; Wichelhausen, R. B.; Roizman, B. Contamination of human cell cultures by pleuropneumonialike organisms. Science 124: 1147–1148; 1956.
Hayflick, L. Decontaminating tissue cultures infected with pleuropneumonia-like organisms. Nature 185: 783–784; 1960.
Pollock, M. E.; Kenny, G. E. Mammalian cell cultures contaminated with PPLO. III. Elimination of PPLO with specific antiserum. Proc. Soc. Exp. Biol. Med. 112: 176–181; 1963.
Friend, C.; Patuleia, M. C.; Nelson, J. B. Antibiotic effect of tylocin on a mycoplasma contaminant in a tissue culture leukemia cell line. Proc. Soc. Exp. Biol. Med. 121: 1009–1010; 1966.
Perlman, D.; Rahman, S. B.; Semar, J. B. Antibiotic control ofMycoplasma in tissue culture. Appl. Microbiol. 15: 82–85; 1967.
Balduzzi, P.; Carbonneau, R. J. Decontamination of pleuropneumonia-like organism (PPLO) infected tissue cultures. Experentia 20: 651–652; 1964.
Barile, M. F.; Grabowski, M. W. Mycoplasma-cell culture-virus interactions: A brief review. McGarrity, G. J.; Murphy, D. G.; Nichols, W. W. eds. Mycoplasma infection of cell cultures. New York: Plenum Press; 1978: 135–150.
Lanks, K. W.; Chin, N. W. Lactoperoxidase-catalyzed iodination of cell cultures infected with mycoplasma. In Vitro 15: 503–506; 1979.
Lombardo, J. M.; Lanks, K. W. Determinants of cultured neuroblastoma cell morphology. Fed. Proc. 37:266; 1978.
Seeds, N. W.; Gilman, A. G.; Amano, T.; Nirenberg, M. W. Regulation of axon formation by clonal lines of a neural tumor. Proc. Natl. Acad. Sci. USA 66: 160–167; 1970.
Chen, T. R.In situ detection ofmycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp. Cell Res. 104: 255–262; 1977.
van Diggelen, O. P.; Shin, S.; Phillips, D. M. Reduction in cellular tumorigenicity after mycoplasma infection and elimination of mycoplasma from infected cultures by passage in nude mice. Cancer Res. 37: 2680–2687; 1977.
Marcus, M.; Avi, V.; Nattenberg, A.; Rottem, S.; Markowitz, O. Selective killing of mycoplasma from contaminated mammalian cells in cell culture. Nature 285: 659–661; 1980.
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This work was supported in part by Grants ES01530, GM24592, and GM26167 from the National Institutes of Health, Bethesda, MD.
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Lombardo, J.M., Lanks, K.W. Elimination ofM. hyorhinis from murine neuroblastoma cell lines by in vivo passage. In Vitro 18, 251–253 (1982). https://doi.org/10.1007/BF02618578
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DOI: https://doi.org/10.1007/BF02618578