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The development of mouse spinal cord in tissue culture

II. Development of neuronal precursor cells

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Summary

Neural tubes of mouse embryos at Theiler Stages 14, 15, and 16 were grown in cultures for 21 d with 0.5 μCi/ml tritiated thymidine or cold growth medium. It was found that 50 to 60% of the neurons formed in the outgrowth zone were labeled, indicating that they formed from precursor cells that proliferated in the cultures. The unlabeled neurons must have formed from cells that were already postmitotic when the cultures were started. By comparing the total number of neurons per neuromere formed in vivo and in vitro, it seems that the postmitotic precursor cells survive better in cultures and only a small percentage of proliferative precursor cells in cultures enter the postmitotic stage and form neurons.

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Authors and Affiliations

  1. Department of Anatomy, University of Saskatchewan, S7N 0W0, Saskatton, Saskatchewan, Canada

    B. H. J. Juurlink & S. Fedorff

Authors
  1. B. H. J. Juurlink
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  2. S. Fedorff
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This work was supported by Grant MT4235 from the Medical Research Council of Canada.

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Juurlink, B.H.J., Fedorff, S. The development of mouse spinal cord in tissue culture. In Vitro 18, 179–182 (1982). https://doi.org/10.1007/BF02618569

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  • DOI: https://doi.org/10.1007/BF02618569

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