Summary
A simple, rapid procedure for the quantitation of the histochemical stain for Δ5-3β-dehydrogenase (3β-HSD) in cultured cells is described. Adrenocortical cells were stained for 3β-HSD and then solubilized with 0.5N NaOH. The absorbance of the solubilized cell solution was measured at 570 nm and a linear relationship was obtained between the number of cells and the intensity of the absorption. It is shown that the regulation of 3β-HSD activity in primary cultures of normal rat adrenocortical cells can be monitored by the quantitative histochemical assay. The results show a good correlation with the data obtained by a biochemical assay for the enzyme.
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This work was supported by National Cancer Institute Grant CA-16417.
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Rybak, S.M., Ramachandran, J. Primary culture of normal rat adrenocortical cells. In Vitro 17, 605–611 (1981). https://doi.org/10.1007/BF02618459
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DOI: https://doi.org/10.1007/BF02618459