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Regulation of endothelial cell DNA synthesis and adherence

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Summary

A defined medium has been developed for primary culture of cells from human umbilical vein that will support maximal levels of cell division. The role of medium components in regulating the amount of thymidine incorporation has been assessed; insulin and to a lesser extent fibroblast growth factor (FGF) both increased the rate of incorporation when hydro-cortisone (HC) was present in the medium. Although these hormones in nonserum medium can stimulate incorporation, plating and maintenance of cells in serum medium for 12 h is necessary before transfer to defined medium. Without serum for this period, cells placed in defined medium, though well attached, did not divide. From the pulse: chase experiments it appears that more than one round of replication was supported by the 12-h period in serum.

The role of various agents in regulating cell adhesion also was assessed. Factors precent in serum but not in platelets appear active. Cold insoluble globulin (CIG) is an active serum component inasmuch as it caused adherence when added to defined medium. However, other serum components were highly effective in promoting adhesion in the absence of CIG. Insulin also induced adhesion in nonserum medium though to a smaller extent; its effect was enhanced by plating cells on collagen. Hydrocortisone potentiated the effect of insulin and caused enhanced cell spreading in serum or CIG containing medium but not other medium. All well-spread cells were capable of fibronectin (FN) synthesis whether in serum or nonserum medium. Neither insulin nor HC stimulated fibronectin synthesis.

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Authors and Affiliations

  1. Department of Pathology, University of California, 90024, Los Angeles, California

    Judith A. Berliner

  2. Laboratory of Nuclear Medicine and Radiation Biology, University of California, 90024, Los Angeles, California

    Judith A. Berliner

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  1. Judith A. Berliner
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This research was supported by a grant from the American Diabetes Association.

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Berliner, J.A. Regulation of endothelial cell DNA synthesis and adherence. In Vitro 17, 985–992 (1981). https://doi.org/10.1007/BF02618423

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  • DOI: https://doi.org/10.1007/BF02618423

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