Skip to main content
Log in

Growth and embryo formation in wild-carrot suspension cultures with ammonium ion as a sole nitrogen source

  • Published:
In Vitro Aims and scope Submit manuscript

Summary

Wild-carrot (Daucus carota L.) suspension cultures grew and produced embryos on ammonium ion as a sole source of nitrogen in the absence of any exogenous Kreb's cycle acid when the pH of the medium was controlled by continuous titration with KOH or KHCO3.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Wetherell, D. F., and D. K. Dougall. 1976. Sources of nitrogen supporting growth and embryogenesis in cultured wild carrot tissue. Physiol. Plant. 37: 97–103.

    Article  CAS  Google Scholar 

  2. Martin, S. M., and D. Rose. 1976. Growth of plant cells (Ipomoea) suspension cultures at controlled pH levels. Can. J. Bot. 54: 1264–1270.

    CAS  Google Scholar 

  3. Bayley, J. M., J. King, and O. L. Gamborg. 1972. The effect of the source of inorganic nitrogen on growth and enzymes of nitrogen assimilation in soybean and wheat cells in suspension cultures. Planta 105: 15–24.

    Article  CAS  Google Scholar 

  4. Rose, D., and S. M. Martin. 1975. Effect of ammonium on growth of plant cells (Ipomoea sp.) in suspension cultures. Can. J. Bot. 53: 1942–1949.

    CAS  Google Scholar 

  5. Gamborg, O. L., and J. P. Shyluk. 1970. The culture of plant cells with ammonium salts as the sole nitrogen source. Plant Physiol. 45: 598–600.

    PubMed  CAS  Google Scholar 

  6. Behrend, J., and R. I. Mateles. 1975. Nitrogen metabolism in plant cell suspension cultures. I. Effect of amino acids on growth. Plant Physiol. 56: 584–589.

    PubMed  CAS  Google Scholar 

  7. Behrend, J., and R. I. Mateles. 1976. Nitrogen metabolism in plant cell suspension cultures. II. Role of organic acids during growth on ammonia. Plant Physiol. 58: 510–512.

    PubMed  CAS  Google Scholar 

  8. Polacco, J. C. 1976. Nitrogen metabolism in soybean tissue culture. I. Assimilation of urea. Plant Physiol. 58: 350–357.

    PubMed  CAS  Google Scholar 

  9. Oaks, A. 1974. The regulation of nitrate reductase in suspension cultures of soybean cells. Biochim. Biophys. Acta 372: 122–126.

    PubMed  CAS  Google Scholar 

  10. Dougall, D. K. 1974. Evidence for the presence of glutamate synthase in extracts of carrot cell cultures. Biochem. Biophys. Res. Commun. 58: 639–646.

    Article  PubMed  CAS  Google Scholar 

  11. Verma, D. C., and D. K. Dougall. 1977. Influence of carbohydrates on quantitative aspects of growth and embryo formation in wild carrot suspension cultures. Plant Physiol. 59: 81–85.

    Article  PubMed  CAS  Google Scholar 

  12. Brown, S., D. F. Wetherell, and D. K. Dougall. 1976. The potassium requirement for growth and embryogenesis in wild carrot suspension cultures. Physiol. Plant. 37: 73–79.

    Article  CAS  Google Scholar 

  13. Verma, D. C., and D. K. Dougall. 1977. DNA, RNA and protein content of tissue during growth and embryogenesis in wild carrot suspension cultures. In Vitro 14: 183–191.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dougall, D.K., Verma, D.C. Growth and embryo formation in wild-carrot suspension cultures with ammonium ion as a sole nitrogen source. In Vitro 14, 180–182 (1978). https://doi.org/10.1007/BF02618220

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02618220

Key words

Navigation