Conclusion
We have reviewed some of our experiences in developing techniques for studying the functions of the cells of the immune system. It is quite clear that much remains to be done. Improvements in the culture system are needed to permit cells to be grown for longer durations and at lower cell concentrations. The important effects of fetal calf serum should be defined. More sophisticated methods for separating cells into distinct functional populations must be developed. New assays for identifying other functions of the cells, particularly a method for directly assaying the number of precursor cells in a population, are needed. When these techniques are applied to the study of immune cells, further facts should be learned which will permit the development of significant, testable hypotheses on the function and relationships of the cells of the immune system.
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This is publication No. 298 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037.
This work was supported in part by U.S.P.H.S. Grant 7007 and in part by American Cancer Society Grant E-395.
Dr. Mishell is supported by American Cancer Society Grant E-395.
Dr. Dutton is supported by a Dernham Fellowship of the California Division, American Cancer Society (No. D-100).
Dr. Raidt is supported by United States Public Health Service Postdoctoral Fellowship No. 7-F2-A1-31,590.
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Mishell, R.I., Dutton, R.W. & Raidt, D.J. Methods for the study ofin vitro immunization of mouse spleen cells. In Vitro 4, 83–91 (1969). https://doi.org/10.1007/BF02618213
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DOI: https://doi.org/10.1007/BF02618213