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An experimental analysis of factors affecting the localization of embryonic bone marrow

  • Part I Hemopoietic Precursors
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Summary

The present investigations have been concerned with factors which determine and influence the localization and development of hemopoietic bone marrow in the embryo mouse and the adult. These studies, which have employed organ cultures and the transplantation of mouse embryo femur and tail rudiments, indicate that the surrounding mesenchyme is required for the normal development of the cartilage rudiment and its ossification, and for the formation and colonization of the marrow cavity. It was suggested that hemopoiesis results from the colonization of the “prepared” marrow cavity by stem cells arising from sources external to the rudiment. The addition of erythropoietin and L-thyroxine produced distinct erythropoietic differentiation in the normally myelocytic embryonic marrow cavity. The significance of the microenvironment present in developing bone rudiments and the initiation of hemopoiesis in stem cells was discussed.

A hypothesis was developed to explain marrow localization in adults based on the colonization of bone rudiments which are developing their marrow sites at a time when the blood contains large numbers of colony-forming units.

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Author notes

  1. Susetta Pons

    Present address: Laboratorio di Radiobiologia Animale, Casaccia, Comitato Nationale per Energia Nucleari, Rome, Italy

Authors and Affiliations

  1. The Department of Epidemiology and International Health, University of California School of Medicine, San Francisco, California

    Nicholas L. Petrakis, Susetta Pons & Rose E. Lee

  2. Laboratorio di Radiobiologia Animale, Casaccia, Comitato Nationale per Energia Nucleari, Rome, Italy

    Nicholas L. Petrakis

Authors
  1. Nicholas L. Petrakis
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  2. Susetta Pons
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  3. Rose E. Lee
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Petrakis, N.L., Pons, S. & Lee, R.E. An experimental analysis of factors affecting the localization of embryonic bone marrow. In Vitro 4, 3–13 (1969). https://doi.org/10.1007/BF02618206

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  • DOI: https://doi.org/10.1007/BF02618206

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