Summary
Eukaryotic DNA is functionally divided into thousands of replicons, each of which may be duplicated at a characteristic time within the DNA synthetic (S) period. Our approach toward an understanding of the molecular mechanisms which control orderly eukaryotic DNA synthesis has been: (a) to devise a method of cell synchrony in a suitable tissue culture system wherein all cells in the population enter and traverse the S period with a high degree of synchrony; (b) to determine, utilizing this system, precisely when during the S period critical events and macromolecular syntheses occur; and (c) to examine, by polyacrylamide-gel electrophoresis, the spectrum of proteins which become associated with chromatin during the S period in such a way as to suggest their involvement with DNA synthesis. Possible mechanisms for control are discussed based on the results presented here.
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The work reported in this communication was supported by NCI Grant CA 18612 to A.B.P.
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Hamlin, J.L., Pardee, A.B. Control of DNA synthesis in tissue culture cells. In Vitro 14, 119–127 (1978). https://doi.org/10.1007/BF02618178
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DOI: https://doi.org/10.1007/BF02618178