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An organ culture of postnatal rat liver slices

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Summary

A technique for the organ culture of postnatal and adult rat liver has been developed. Liver slices, 0.3 mm thick, were maintained in Conway units at the interphase between medium and a 95% O2:5% CO2 atmosphere. Postnatal liver in culture for up to 72 h had healthy hepatocytes throughout the explants; if adult liver was used the upper 0.2 mm was healthy after 24 h. These slices incorporated tritiated orotate and leucine into trichloroacetic acid-precipitable material. Incorporation of orotate was shown to be spread over the entire slice of neonatal liver. Culturing did not alter the potassium ion content of postnatal liver. Tyrosine aminotransferase activity in liver slices from postnatal, adult, and adrenalectomized adult rats was stimulated by glucocorticoids and dibutyryl cyclic AMP. Cycloheximide and actinomycin D prevented this response. Further, cortisol exerted a permissive effect on the stimulation of tyrosine aminotransferase activity by dibutyryl cyclic AMP in slices from adrenalectomized rats. Induction of urea cycle enzymes by cortisol was demonstrated in cultures of liver from adrenalectomized adult animals.

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Authors and Affiliations

  1. Department of Biochemistry, University of Witwatersrand, Johannesburg, South Africa

    Adele Hart

  2. Department of Zoology, Iowa State University, 50011, Ames, Iowa

    Frederick J. Mattheyse & John B. Balinsky

Authors
  1. Adele Hart
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  2. Frederick J. Mattheyse
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  3. John B. Balinsky
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Additional information

Deceased October 1, 1983.

This research was supported in part by a grant from the South African Council for Scientific and Industrial Research.

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Hart, A., Mattheyse, F.J. & Balinsky, J.B. An organ culture of postnatal rat liver slices. In Vitro 19, 841–852 (1983). https://doi.org/10.1007/BF02618164

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  • DOI: https://doi.org/10.1007/BF02618164

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