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Binding, internalization, and degradation of [125I]insulin by cultured bovine aortic endothelial cells: Effects of serotonin

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Summary

Cultured bovine aortic endothelial cells bind and internalize[125I]insulin and down regulate insulin receptors. Internalized insulin was not degraded significantly and diffused from the endothelial cells. Neither 5-hydroxytryptamine, methylamine, nor dansyl-cadaverine have any observable effect on insulin binding, internalization, metabolism, or down regulation of insulin receptors. Transglutaminase activity, however, is inhibited by 5-hydroxytryptamine and methylamine. These data indicate that transglutaminase is not required for insulin receptor-mediated endocytosis by bovine aortic endothelial cells in culture.

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Authors and Affiliations

  1. Biological Science Center, Microvascular Research, 2 Cummington St., 02215, Boston, MA

    Scott W. Peterson & David Shepro

  2. Department of Pathology, Microvascular Research, 2 Cummington St., 02215, Boston, MA

    Mary Pat Carson

  3. School of Medicine, Boston University, 02215, Boston, Massachusetts

    Mary Ellen Moynahan

Authors
  1. Mary Pat Carson
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  2. Scott W. Peterson
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  3. Mary Ellen Moynahan
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  4. David Shepro
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Additional information

This work was supported in part by U.S. Public Health Service NHL B 16714-08 and GM 28832-03.

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Carson, M.P., Peterson, S.W., Moynahan, M.E. et al. Binding, internalization, and degradation of [125I]insulin by cultured bovine aortic endothelial cells: Effects of serotonin. In Vitro 19, 833–840 (1983). https://doi.org/10.1007/BF02618163

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  • DOI: https://doi.org/10.1007/BF02618163

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