Summary
A vessel consisting of a Teflon well former, metal base plate, rubber gasket, and variable growth surface has been constructed. It is nontoxic, autoclavable, and reusable. It provides for ready access into the growth chamber, plus an optically clear lower surface for improved microscopic resolution. The vessel has been used successfully with several different eucaryotic and procaryotic cell systems. Morphology of cells and yields (as measured by total protein and ATP) are comparable to those noted with other vessels. This Chamber/Dish can accommodate almost any type of growth substrate in the form of films or sheets. It appears to have widespread application for the growth of cells and explants in vitro.
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Patent applied for. Chamber/Dish, Bionique, P.O. Box 535, Lake Placid, NY 12946.
This work was supported in part by a special grant from the Bellco Glass Company and NIH Research Grant A1-12559. The author acknowledges the expert technical assistance of R. A. Meccoli, L. Hoglund, and S. Upchurch, along with the machinists of the Biology Shop/School of Life Sciences, University of Illinois at Urbana. Cell lines were generously supplied by C. Bergholz and P. Wong.
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Gabridge, M.G. The Chamber/Dish: An improved vessel for cell and explant culture. In Vitro 17, 91–97 (1981). https://doi.org/10.1007/BF02618064
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DOI: https://doi.org/10.1007/BF02618064