Summary
A modified method for establishing cultures of rabbit corneal cells is described. The new technique utilized a Lucite disc in combination with a Tygon ring for growth of pure cell cultures and was compared with an explant method for growing cells. Each method provided adequate cell cultures for biochemical or ultrastructure studies of rabbit corneal cells, but the ring and disc method described here allowed the isolation of specific cell types without the interference of stromal cell contamination.
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References
Mannagh, J., and A. R. Irving. 1965. Human corneal endothelial growth in tissue cultures. Arch. Opthalmol. 74: 844–851.
Slick, W. C., J. Mannagh, and Z. Yuhasy. 1965. Enzymatic removal of pure culture of rabbit corneal endothelial cells. Arch. Opthalmol. 73: 229–232.
Manski, W., and T. L. Whiteside. 1974. Cell-surface receptors of normal, regenerating and cultured corneal epithelial and endothelial cells. Invest. Opthalmol. 10:935–941.
Newsome, D. A., M. Takasugi, K. R. Kenyon, W. F. Stark, and G. Opelz. 1974. Human corneal cells in vitro: morphology and histocompatibility (HL-A) antigens of pure cell populations. Invest. Opthalmol. 13: 23–28.
Perlman, M., and J. L. Baum. 1974. The mass culture of rabbit corneal endothelial cells. Arch. Ophthalmol. 92: 235–239.
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Glassman, A.B., Coles, W.H. & Bennett, C.E. Corneal endothelium: A modified method for cultivation. In Vitro 15, 873–876 (1979). https://doi.org/10.1007/BF02618043
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DOI: https://doi.org/10.1007/BF02618043