Summary
The induction of HeLa cell alkaline phosphatase activity by sodium butyrate could be inhibited by the coadministration of caffeine or theophylline. The inhibitions were dose dependent, and at any given concentration the potency was theophylline > caffeine. Although the induction by sodium butyrate was more sensitive to the inhibition by the xanthines than was that produced by 5-iodo-2′-deoxyuridine, the magnitudes of the increases in cyclic AMP concentrations after treatment with the xanthines were similar in the inhibition of both types of induction. The induction of alkaline phosphatase activity by sodium butyrate also produced a shift in the thermostability pattern of the enzyme, with a proportionately greater increase in the heat-labile, rather than heat-stable, from of the activity.
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Cox, R. P., and C. M. MacLeod. 1962. Alkaline phosphatase content and the effects of prednisolone on mammalian cells in culture. J. Gen. Physiol. 45: 439–485.
Goz, B. 1974. The induction of alkaline phosphatase activity in HeLa cells by 5-iodo-2′ deoxyuridine. Cancer Res. 34: 2393–2398.
Patrick, J. C., Jr., S. Rengachary, and G. Melnykovych. 1975. Elevation of adenosine 3′5′-cyclic monophosphate in established mammalian cell strains by Hypaque (sodium diatrizoate). In Vitro 11: 404–408.
Wharton, W., and B. Goz. 1978. Induction of alkaline phosphatase activity in HeLa cells by choline chloride. Cancer Res. 38: 3764–3768.
Griffin, M. J., G. H. Price, K. L. Bazzell, R. P. Cox, and N. K. Ghosh. 1974. A study of adenosine 3′,5′-cyclic monophosphate, sodium butyrate and cortisol as inducers of HeLa alkaline phosphatase. Arch. Biochem. Biophys. 164: 619–623.
Wharton, W., and B. Goz. 1979. Induction, of alkaline phosphatase activity in HeLa cells. Inhibition by xanthine derivatives and thermostability studies. Biochem. Pharmac. 28: 763–768.
Wharton, W., and B. Goz. 1978. Cyclic AMP concentrations in HeLa cells during inhibition of alkaline phosphatase activity by xanthine phosphodiesterase inhibitors. Pharmacologist. 20: 147.
Herz, F., and H. M. Nitowsky. 1962. Alkaline phosphatase activity of human cell cultures: kinetic and physical-chemical properties. Arch. Biochem. Biophys. 96: 506–515.
Melnykovych, G., and C. K. Sylber. 1966. Effects of cations on activity and thermostability of alkaline phosphatase in HeLa cells. Proc. Soc. Exp. Biol. Med. 121: 165–168.
Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265–275.
Krishna, G., B. Weiss, and B. B. Brodie. 1968. A simple, sensitive method for the measurement of adenyl cyclase. J. Pharmacol. Exp. Ther. 163: 379–385.
Steiner, A. L., C. W. Parker, and D. M. Kipnis. 1977. Radioimmunoassay for cyclic nucleotides. I. Preparation of antibodies and iodinated cyclic nucleotides. J. Biol. Chem. 247: 1106–1113.
Riggs, M. G., R. G. Whittaker, J. R. Neumann, and V. M. Ingram. 1977.n-Butyrate causes histone modification in HeLa and Friend erythroleukaemia cells. Nature 268: 462–464.
Tallman, J. F., C. C. Smith, and R. C. Henneberry. 1977. Induction of functional beta-adrenergic receptors in HeLa cells. Proc. Nat. Acad. Sci. U.S.A. 74: 873–877.
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Supported by National Cancer Institute Grant CA16460.
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Wharton, W., Hart, C.A. & Goz, B. Induction of alkaline phosphatase activity in HeLa cells by sodium butyrate. In Vitro 15, 861–864 (1979). https://doi.org/10.1007/BF02618041
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DOI: https://doi.org/10.1007/BF02618041