Summary
Ribosomal ribonucleic acids (rRNA's) of cultured cells from various species were compared by polyacrylamide gel electrophoresis. Electrophoretic mobility of the 28 S RNA component varied according to species. Human cell 28 S rRNA was distinguishable from that of monkey cells. The mobility of chimpanzee 28 S rRNA was identical to that from human cells, but different from that of monkey cells. Cat, goat, and swine cell 28 S rRNA's were distinguishable from one another and from both human and monkey cell rRNA's. The mobilities of bovine, rabbit, rat, hamster, and mouse cell 28 S rRNA's were virtually identical to that from monkey cells. No apparent correlation existed between the mobility of 28 S rRNA and the currently accepted phylogenetic position of the donors. Normal cells, tumor cells, and virus-infected cells from the same species did not differ, but in some instances embryonic and adult cells were distinguishable. The 18 S rRNA components did not show variations comparable to those of the 28 S rRNA components.
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References
Loening, U. E. 1968. Molecular weights of ribosomal RNA in relation to evolution. J. Mol. Biol. 38: 355–365.
Loening, U. E. 1970. The mechanism of synthesis of ribosomal RNA. Symp. Soc. Gen. Microbiol. 20: 77–106.
Schaffer, F. L., M. E. Soergel, and D. C. Straube 1971. Electrophoretic analysis of ribosomal and viral ribonucleic acids with a simple technique for slicing low-concentration polyacrylamide gels. Appl. Microbiol. 22: 538–545.
Loening, U. E. 1969. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel acrylamide-gel electrophoresis. Biochem. J. 113: 131–138.
Fisher, M. P., and C. W. Dingman. 1971. Role of molecular conformation in determining the electrophoretic properties of polynucleotides in agarose-acrylamide composite gels. Biochemistry 10: 1895–1899.
Staynov, D. Z., J. C. Pinder, and W. B. Gratzer. 1972. Molecular weight determination of nucleic acids by gel electrophorosis in non-aqueous solution. Nature New Biol. 235: 108–110.
Morton, H. J. 1970. A survey of commercially available tissue culture media. In Vitro 6: 89–108.
Field, A. K., and J. Fong. 1964. Mouse salivary gland virus plaque assay on a stable line of mouse embryo cells. J. Bacteriol. 87: 1238–1239.
McAllister, R. M., W. A. Nelson-Rees, E. Y. Johnson, R. W. Rongey, and M. B. Gardner. 1971. Disseminated rhabdomyosarcomas formed in kittens by cultured human rhabdomyosarcoma cells. J. Natl. Cancer Inst. 47: 603–612.
McClure, H. M., K. H. Belden, and W. A. Pieper. 1969. Autosomal trisomy in a chimpanzee: resemblance to Down's syndrome. Science 165: 1010–1012.
Jensen, E. M., I. Zelljadt, H. C. Chopra, and M. M. Mason. 1970. Isolation and propagation of a virus from a spontaneous mammary carcinoma of a rhesus monkey. Cancer Res. 30: 2388–2393.
Ahmed, M., S. A. Mayyasi, H. C. Chopra, I. Zelljadt, and E. M. Jensen. 1971. Mason-Pfizer monkey virus isolated from spontaneous mammary carcinoma of a female monkey. I. Detection of virus antigens by immunodiffusion, immunofluorescent, and virus agglutination techniques. J. Natl. Cancer Inst. 46: 1325–1334.
Nelson-Rees, W. A., L. E. Hooser, and A. J. Hackett. 1972. Chronic poliovirus infection of co-cultivated monkey cells (CMMT) harboring the Mason-Pfizer monkey virus (M-PMV). J. Natl. Cancer Inst. In press.
Sykes, J. A., J. Whitescarver, and L. Briggs. 1968. Observations on a cell line producing mammary tumor virus. J. Natl. Cancer Inst. 41: 1315–1327.
Hsu, T. C., and K. Benirschke. 1967.An Atlas of Mammalian Chromosomes. Vol. 1. Springer-Verlag, New York.
Rothfels, K. H. and L. Siminovitch. 1958. An air drying technique for flattening chromosomes in mammalian cells grownin vitro. Stain Technol. 33: 73–77.
Markov, G. G., I. Bradvarova, A. Mintcheva, P. Petrov, N. Shishkov, and R. G. Tsanev. 1969. Mycoplasma contamination of cell cultures. Interference with32P-labelling pattern of RNA. Exp. Cell Res. 57: 374–384.
Hackett, A. J. 1964. A possible morphologic basis for the autointerference phenomenon in vesicular stomatitis virus. Virology 24: 51–59.
Borun, T. W., M. D. Scharff, and E. Robbins. 1967. Preparation of mammalian polyribo-somes with the detergent Nonidet P-40. Biochim. Biophys. Acta 149: 302–304.
Schaffer, F. L., and M. E. Soergel. 1972. Molecular weight estimates of vesicular stomatitis virus ribonucleic acids from virions, defective particles, and infected cells. Manuscript in preparation.
Herrick, P. R., G. W. Baumann, D. J. Merchant, M. C. Shearer, C. Shipman, Jr., and R. G. Brackett. 1970. Serologic and karyologic evidence of incorrect identity of an animal cell line (guinea pig spleen). In Vitro 6: 143–147.
Montes De Oca, F., M. L. Macy, and J. E. Shannon. 1969. Isoenzyme characterization of animal cell cultures. Proc. Soc. Exp. Biol. Med. 132: 462–469.
Fogh, J., N. B. Holmgren, and P. P. Ludovici. 1971. A review of cell culture contaminations. In Vitro 7: 26–41.
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This work was supported by the Office of Naval Research and the Bureau of Medicine and Surgery, United States Navy, under a contract between the Office of Naval Research and the Regents of the University of California.
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Soergel, M.E., Schaffer, F.L. Ribosomal ribonucleic acids of cultured cells: A preliminary survey of differences among mammalian species detectable by polyacrylamide gel electrophoresis. In Vitro 8, 13–18 (1972). https://doi.org/10.1007/BF02617938
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DOI: https://doi.org/10.1007/BF02617938