Effect of activated charcoal on callus growth and shoot organogenesis in tobacco

Summary

Incorporating activated charcoal (AC) in culture media has been shown to affect growth and development of various organisms. Since AC stimulates the development of tobacco haploid plantlets from cultured anthers, research was conducted to determine the effect of activated charcoal on pith-derived callus growth and shoot development inNicotiana tabacum cv. Wisconsin 38. Our results indicate that the hormones required for callus growth and shoot development in Wisconsin-38 tobacco are adsorbed by AC, thereby inhibiting callus growth and prohibiting shoot development. This effect was observed even when AC was removed from the medium by filtration prior to culturing the callus.

This is a preview of subscription content, access via your institution.

References

  1. 1.

    Anagnostakis, S. L. 1974. Haploid plants from anthers of tobacco—enhancement with charcoal. Planta 115:281–283.

    Article  Google Scholar 

  2. 2.

    Nakamura, A., and R. Itagaki. 1973. Anther culture inNicotiana and the characteristics of the haploid plants. Jpn. J. Breed. 23:71–78.

    Google Scholar 

  3. 3.

    Fridborg, G., and T. Eriksson. 1975. Effects of activated charcoal on growth and morphogenesis in cell cultures. Physiol. Plant 32:306–308.

    Article  Google Scholar 

  4. 4.

    Ernst, R. 1974. The use of activated charcoal in a symbiotic seedling culture ofPaphiopedilum. Am. Orchid Soc. Bull. 43:35–38.

    Google Scholar 

  5. 5.

    Wang, P. J., and L. C. Huang. 1976. Beneficials effects of activated charcoal on plant tissue and organ cultures. In Vitro 12:260–262.

    PubMed  CAS  Google Scholar 

  6. 6.

    Klein, B., and M. Bopp. 1971. Effect of activated charcoal in agar on the culture of lower plants. Nature 230:474.

    Article  CAS  Google Scholar 

  7. 7.

    Proskauer, J., and R. Berman. 1970. Agar culture medium modified to approximate soil conditions. Nature 227:1161.

    Article  Google Scholar 

  8. 8.

    Day, P. R., and S. L. Anagnostakis. 1971. Corn smut dikaryon in culture. Nature [New Biol.] 231:19–20.

    Article  CAS  Google Scholar 

  9. 9.

    Butler, E. E., and F., Bolkan. 1973. A medium for heterokaryon formation inRhizoctoniasolani. Phytopathology 63:542–543.

    Google Scholar 

  10. 10.

    Murashige, T., and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15:473–497.

    Article  CAS  Google Scholar 

  11. 11.

    Gordon, S. A., and R. P. Weber. 1950. Colorimetric estimation of indoleacetic acid. Plant Physiol. 26:192–195.

    Article  Google Scholar 

  12. 12.

    Reinert, J. 1973. Aspects of organization—organogenesis and embryogenesis. In: H. E. Street (Ed.),Plant Tissue and Cell Culture. University of California Press, Berkeley and Los Angeles, pp. 338–355.

    Google Scholar 

  13. 13.

    Engvild, K. C. 1973. Shoot differentiation in callus cultures ofDatura innoxia. Physiol. Plant 28:155–159.

    Article  CAS  Google Scholar 

  14. 14.

    Engelke, A. L., H. Q. Hamzi, and F. Skoog. 1973. Cytokinin gibberellin regulation of shoot development and leaf form in tobacco plantlets. Amer. J. Bot. 60:491–495.

    Article  CAS  Google Scholar 

  15. 15.

    Mattson, J. S., and J. B. Mark, Jr. 1971.Activated Carbon. Marcel Dekker, New York.

    Google Scholar 

  16. 16.

    Rathbone, M. P. 1976. Personal communication.

Download references

Author information

Affiliations

Authors

Additional information

Research sponsored by the U.S. Energy Research and Development Administration under Contract No. E-40-1-GEN-242 with the University of Tennessee.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Constantin, M.J., Henke, R.R. & Mansur, M.A. Effect of activated charcoal on callus growth and shoot organogenesis in tobacco. In Vitro 13, 293–296 (1977). https://doi.org/10.1007/BF02616173

Download citation

Key words

  • Nicotiana tabacum
  • shoot organogenesis
  • activated charcoal
  • callus