Effect of activated charcoal on callus growth and shoot organogenesis in tobacco


Incorporating activated charcoal (AC) in culture media has been shown to affect growth and development of various organisms. Since AC stimulates the development of tobacco haploid plantlets from cultured anthers, research was conducted to determine the effect of activated charcoal on pith-derived callus growth and shoot development inNicotiana tabacum cv. Wisconsin 38. Our results indicate that the hormones required for callus growth and shoot development in Wisconsin-38 tobacco are adsorbed by AC, thereby inhibiting callus growth and prohibiting shoot development. This effect was observed even when AC was removed from the medium by filtration prior to culturing the callus.

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Research sponsored by the U.S. Energy Research and Development Administration under Contract No. E-40-1-GEN-242 with the University of Tennessee.

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Constantin, M.J., Henke, R.R. & Mansur, M.A. Effect of activated charcoal on callus growth and shoot organogenesis in tobacco. In Vitro 13, 293–296 (1977). https://doi.org/10.1007/BF02616173

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Key words

  • Nicotiana tabacum
  • shoot organogenesis
  • activated charcoal
  • callus