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Spontaneous51Cr release by isolated rat hepatocytes: An indicator of membrane damage

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Summary

Radiochromium uptake and release by isolated rat hepatocytes in suspension was monitored under continuous-labeling conditions. Cell protein remained unchanged during the absorption phase, whereas the release of51Cr correlated well with the loss of cell viability and release of cytoplasmic protein. The results suggest that under equilibrium conditions,51Cr release represents an efflux of label from damaged or dying preparations and not an elution of radioisotope from intact cells.

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  1. Faculty of Pharmacy, University of Toronto, M5S 1A1, Toronto, Ontario, Canada

    R. Zawydiwski & G. R. Duncan

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  1. R. Zawydiwski
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  2. G. R. Duncan
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Zawydiwski, R., Duncan, G.R. Spontaneous51Cr release by isolated rat hepatocytes: An indicator of membrane damage. In Vitro 14, 707–714 (1978). https://doi.org/10.1007/BF02616167

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