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Growth of callus and suspension culture cells from cotton varieties (Gossypium hirsutum L.) resistant and susceptible toxanthomonas malvacearum (E. F. Sm.) dows

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Summary

In vitro conditions are defined for starting and maintaining callus and suspension, cells from two cotton (Gossypium hirsitum L.) varieties, Im 216 and Acala 44, which are resistant and susceptible, respectively, to the bacterial pathogenXanthomonas malvacearum (E. F. Sm.) Dows. A light, friable callus was easily obtained and has been maintained for over 4 years. Whether stems or leaves, the explant source for callus initiation made no difference for growth of callus tissue. Acala 44 callus had a fresh-weight doubling time of 4 to 5 days, and Im 216 callus had a fresh-weight doubling time of 4 to 9 days; however, in suspension culture the fresh-weight doubling times for Im 216 and Acala 44 were 6 days. The pH of the suspension medium dropped to 4.7 during the exponential growth phase and rose to 5.4 at the stationary phase. Attempts to induce root and shoot initiation from these callus cells were unsuccessful; however, greening of the callus tissue did occur. Schenk and Hildebrandt medium was used for both callus and suspension cultures. Inoculation of Im 216 and Acala 44 callus tissues with two races ofX. malvacearum resulted in a resistant and susceptible response, respectively.

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Authors and Affiliations

  1. Department of Biochemistry, Oklahoma Agricultural Experiment Station, Oklahoma State University, 74074, Stillwater, Oklahoma

    John Ruyack, Michael R. Downing, Judy Su Chang & Earl D. Mitchell Jr.

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  1. John Ruyack
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  2. Michael R. Downing
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  3. Judy Su Chang
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  4. Earl D. Mitchell Jr.
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This research was supported in part by C. S. R. S. Grant 315-16-96 and the Agricultural Experiment Station of Oklahoma State University.

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Ruyack, J., Downing, M.R., Chang, J.S. et al. Growth of callus and suspension culture cells from cotton varieties (Gossypium hirsutum L.) resistant and susceptible toxanthomonas malvacearum (E. F. Sm.) dows. In Vitro 15, 368–373 (1979). https://doi.org/10.1007/BF02616144

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