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Metabolism of juvenile hormone in cultures of ovaries and fat body in the cockroachperiplaneta Americana

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Summary

The time course of juvenile hormone (JH) metabolism is examined in cultures ofPeriplaneta americana fat body and ovaries in medium containingManduca sexta carrier protein or cockroach hemolymph. In the absence ofM. sexta carrier protein or cockroach hemolymph, both tissues extensively catabolize exogenous [3H]JH in the medium. Addition of the carrier protein or hemolymph to the culture system prevents the hydrolysis of the hormone in the medium. Within the tissues JH is degraded whether or not carrier protein or hemolymph is present which suggests that the protective role of these molecules is exclusively extracellular. Incubation of [3H]JH with medium preconditioned with tissue results in destruction of the hormone. This suggests that the fat body secretes esterases into the medium. In contrast, the ovarioles hydrolyze the hormone by means of cell-associated enzyme. The relationship of these phenomena to insect development is discussed.

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Author notes

  1. Gary R. Sams

    Present address: Department of Physiological Chemistry, University of Wisconsin, 53706, Madison, Wisconsin

Authors and Affiliations

  1. Department of Physiology and Cell Biology, University of Kansas, 66045, Lawrence, Kansas

    Gary R. Sams, Gerald F. Cocchiaro & William J. Bell

  2. Department of Entomology, University of Kansas, 66045, Lawrence, Kansas

    Gary R. Sams, Gerald F. Cocchiaro & William J. Bell

Authors
  1. Gary R. Sams
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  2. Gerald F. Cocchiaro
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  3. William J. Bell
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Additional information

This work supported by NSF Grant PCM 76-02229 and University of Kansas Biomedical Sciences Grant RR-07037.

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Sams, G.R., Cocchiaro, G.F. & Bell, W.J. Metabolism of juvenile hormone in cultures of ovaries and fat body in the cockroachperiplaneta Americana . In Vitro 14, 956–960 (1978). https://doi.org/10.1007/BF02616127

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