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Effects of theophylline, epidermal chalone and X-irradiation on proliferation and differentiation of human keratinocytes in vitro

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Outgrowth cultures of normal human epidermis were used to study a possible relationship between growth inhibition and differentiated function. The effects of theophylline, epidermal chalone and x-irradiation on mitoses and the characteristic production of epidermal keratohyaline granules (KG) were examined at various intervals after the treatment. Theophylline (an inhibitor of cyclic nucleotide phosphodiesterase) or epidermal chalone inhibited mitoses and enhanced KG production. X-irradiation inhibited mitoses but had no effect on KG formation. These results indicate that inhibition of proliferation per se is not sufficient to enhance keratinization of human epidermal cells.

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Authors and Affiliations

  1. Cell Biology Division, Southern Research Institute, 2000 Ninth Avenue, South, 35205, Birmingham, Alabama

    Dharam P. Chopra

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  1. Dharam P. Chopra
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Additional information

This work was supported by Grant 1-PO-ICA-11563 from the National Cancer Institute and Grant 1PO-1AM-15515 from the National Institute of Arthritis and Metabolic Diseases. The research was carried out at the Skin and Cancer Hospital, Temple University Health Sciences Center, Philadelphia, Pennsylvania.

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Chopra, D.P. Effects of theophylline, epidermal chalone and X-irradiation on proliferation and differentiation of human keratinocytes in vitro. In Vitro 14, 939–944 (1978). https://doi.org/10.1007/BF02616124

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