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Differing effects of isoleucine deficiency on the toxicity of MNNG for 10T1/2 and CHO cells

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Summary

Isoleucine deficiency sensitizes C3H 10T1/2 cells to the cytotoxic effects of MNNG. Synchrony of proliferation is not required for this effect since it occurs prior to full growth arrest and subsequent establishment of synchronous proliferation after refeeding in complete medium. Furthermore, confluence arrest of proliferation of 10T1/2 cells does not affect their cytotoxic response to MNNG, although they proliferate synchronously after replating at low density. In contrast, the toxicity of MNNG for CHO cells is not altered by isoleucine deficiency, even though these cells are readily synchronized by refeeding in complete medium after transient isoleucine deficiency.

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Authors and Affiliations

  1. Department of Pathology, University of North Carolina, 27514, Chapel Hill, North Carolina

    Diane S. Greenberg, Joe W. Grisham, William N. Bell, Mary S. Baker & David G. Kaufman

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  1. Diane S. Greenberg
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  2. Joe W. Grisham
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  3. William N. Bell
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  4. Mary S. Baker
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  5. David G. Kaufman
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This research was supported by Grant BC-142 from the American Cancer Society and Grants CA-16086 and CA-17973 from the National Cancer Institute.

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Greenberg, D.S., Grisham, J.W., Bell, W.N. et al. Differing effects of isoleucine deficiency on the toxicity of MNNG for 10T1/2 and CHO cells. In Vitro 14, 516–521 (1978). https://doi.org/10.1007/BF02616093

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