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A diploid rat liver cell culture

1. Characterization and sensitivity to aflatoxin B1

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Summary

A method for culturing and cloning a cell line derived from rat hepatic tissue is described. The cloned cells in culture were diploid, epithelioid in morphology, and synthesized tyrosine aminotransferase. The cytotoxic effects of the hepatocarcinogen aflatoxin B1 on these cells were compared to the effects on an established cell culture. The diploid culture was found to be more resistant to the toxin as measured by cell counts and residual protein as well as by [14C]uridine incorporation.

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Authors and Affiliations

  1. College of Medicine, The University of Vermont, 05401, Burlington, Vermont

    John Bausher

  2. Department of Medical Microbiology, The University of Vermont, College of Medicine, 05401, Burlington, Vermont

    Warren I. Schaeffer

Authors
  1. John Bausher
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  2. Warren I. Schaeffer
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Additional information

This work was supported by Public Health Service Grant R01-12056-01.

In partial fulfillment for the degree of Doctor of Philosophy.

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Bausher, J., Schaeffer, W.I. A diploid rat liver cell culture. In Vitro 9, 286–293 (1974). https://doi.org/10.1007/BF02616075

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  • DOI: https://doi.org/10.1007/BF02616075

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