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Isolation and growth characteristics of cell lines from bovine venous endothelium

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Summary

Endothelial isolates from excised bovine vena cava were obtained following a 10 min incubation with versene (ethylenediaminetetraacetic acid). From 19 specimens, 14 cell lines with similar in vitro characteristics have been initiated and maintained in continuous culture for over 22 passages. The predominant cell type in these lines possesses morphologic characteristics similar to those of the tissue of origin. Attached to glass cover slips or plastic flasks the cells are polygonal-shaped and form a mosaic-patterned monolayer. When grown in continuous sheets, the shape and arrangement of the cell, as demonstrated by silver nitrate staining, resembles that of ndothelium. Scanning electron micrographs reveal the presence of numerous cytoplasmic projections on the surface of the bovine endothelial cells. Projections of similar morphology are seen on the surface of the cultured cells. That the in vitro lines actually represent endothelial cells or endothelial precursors is suggested but not proven by these morphologic observations. Evaluation of the functional capabilities of these cells is necessary to determine their true nature.

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This reseach was supported by the Medical Devices Applications Program under United States Public Health Services Contract NHLI-71-2060 from the National Institutes of Health

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Wechezak, A.R., Mansfield, P.B. Isolation and growth characteristics of cell lines from bovine venous endothelium. In Vitro 9, 39–45 (1973). https://doi.org/10.1007/BF02615988

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