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Karyological and biochemical evidence for chromosomal dedifferentiation in neoplasia

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Summary

Quantitative analysis of the X-linked enzyme, glucose 6-phosphate dehydrogenase (G-6-PD), was performed on tumor cells lines from two human females. Both tumor cells were hyperdiploid, having complete or redundant C+X groups. One, no. 930, lacked the X chromatin body and exhibited twice the level of G-6-PD as in the X chromatin-positive tumor cells, ME-180. Hence, in the no. 930 cell, reversal of X chromosomal condensation was associated with loss of the X chromatin body and doubling of genetic activity.

Cells of no. 930 were subsequently placed in culture where after three passages they developed an X chromatin body (or bodies). G-6-PD determinations made at that time showed enzyme levels comparable to the X chromatin-positive tumor cells (ME-180).

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References

  1. Beutler, E., M. Yeh, and V. F. Fairbanks. 1962. The normal human female as a mosaic of X chromosome activity: Studies using the gene for G-6-PD-deficiency as a marker. Proc. Natl. Acad. Sci. U.S.A. 48: 9–16.

    Article  PubMed  CAS  Google Scholar 

  2. Beutler, E., and M. C. Baluda. 1964. The separation of glucose-6-phosphate dehydrogenase deficient erythrocytes from the blood of heterozygotes for glucose-6-phosphate-dehydrogenase deficiency. Lancet i: 189–192.

    Article  Google Scholar 

  3. Davidson, R. G., H. M. Nitowsky, and B. Childs. 1963. Demonstration of cells in human female heterozygous for glucose-6-phosphate-dehydrogenase variants. Proc. Natl. Acad. Sci. U.S.A. 50: 481–485.

    Article  PubMed  CAS  Google Scholar 

  4. Migeon, B. R., V. M. Kaloustian, L. W. Nyhan, W. D. Young, and B. Childs. 1968. X-linked hypoxanthine-guanine phosphoribosyl transferase deficiency heterozygote has two clonal populations. Science 160: 425–427.

    Article  PubMed  CAS  Google Scholar 

  5. Ohno, S., and S. Makino. 1961. The single X nature of the sex chromatin in man. Lancet i: 78–79.

    Article  Google Scholar 

  6. Klinger, H. P., J. Davis, P. Goldhuber, and T. Ditta. 1968. Factors influencing mammalian X-chromosome condensation and sex chromatin formation. Cytogenetics 7: 39–57.

    PubMed  CAS  Google Scholar 

  7. Comings, D. E. 1967. Sex chromatin, nuclear size and the cell cycle. Cytogenetics 6: 120–144.

    PubMed  CAS  Google Scholar 

  8. Straub, D. G., L. A. Lucas, N. J. McMahon, O. L. Pellett, and R. L. Teplitz. 1969. Apparent reversal of X-condensation mechanism in tumors of the female. Cancer Res. 29: 1233–1243.

    PubMed  CAS  Google Scholar 

  9. Sykes, J. A., J. Whitescarver, P. Jernstrom, J. F. Nolan, and P. Byatt. 1970. Some properties of a new epithelial cell line of human origin. J. Natl. Cancer Inst. 45: 107–122.

    PubMed  CAS  Google Scholar 

  10. Zinkham, W. H., M. R. E. Lenhard, and B. Childs. 1958. A deficiency of glucose-6-phosphate-dehydrogenase acitivity in erythrocytes from patients with favism. Bull. Johns Hopkins Hosp. 102: 169–175.

    PubMed  CAS  Google Scholar 

  11. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275.

    PubMed  CAS  Google Scholar 

  12. Teplitz, R. L., K. M. Hansson, V. L. Foley, and M. R. Teplitz. 1966. A model for study of autoimmune disease and its relationship to karyokinesis and cytomorphology. Exp. Cell Res. 41: 686–688.

    Article  PubMed  CAS  Google Scholar 

  13. Ross, A.. 1960. The demonstration of sex chromatin in buccal smears. J. Med. Lab. Technol. 17: 178.

    PubMed  CAS  Google Scholar 

  14. Cook, P. R.. 1970. Species specificity of an enzyme determined by an erythrocyte nucleus in an interspecific hybrid cell. J. Cell Sci. 7: 1–3.

    PubMed  CAS  Google Scholar 

  15. Sieger, M., F. Pera, and H. G. Schwarzacher. 1970. Genetic inactivity of heterochromatin and heteropyknosis inMicrotus agrestis. Chromosoma 29: 349–364.

    Article  PubMed  CAS  Google Scholar 

  16. Klevecz, R. R. 1969. Temporal coordination of DNA replication with enzyme synthesis in diploid and heteroploid cells. Science 166: 1536–1538.

    Article  CAS  Google Scholar 

  17. Bottomley, R. H., A. L. Trainer, and M. J. Griffin. 1969. Enzymatic and chromosomal characterization of HeLa variants. J. Cell Biol. 41: 806–815.

    Article  PubMed  CAS  Google Scholar 

  18. Berman, P. H., M. E. Balis, and J. Dancis. 1968. Diagnostic test for congenital hyperuricemia with central nervous system dysfunction. J. Lab. Clin. Med. 71: 247–253.

    PubMed  CAS  Google Scholar 

  19. Barr, M. L., and E. G. Bertram. 1961. A morphological distinction between neurones of the male and female, and the behaviour of nucleolar satellite during accelerated nucleoprotein synthesis. Nature (Lond.) 163: 78–79.

    Google Scholar 

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Authors and Affiliations

  1. Department of Cytogenetics and Cytology, City of Hope National Medical Center, 91010, Duarte, California

    Raymond L. Teplitz, Kenneth J. Barr & Helen J. Lawce

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  1. Raymond L. Teplitz
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  2. Kenneth J. Barr
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  3. Helen J. Lawce
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This research was supported by United States Public Health Service Grant CA 08791-03.

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Teplitz, R.L., Barr, K.J. & Lawce, H.J. Karyological and biochemical evidence for chromosomal dedifferentiation in neoplasia. In Vitro 7, 195–200 (1972). https://doi.org/10.1007/BF02615975

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