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Establishment of lymphocytoid cell lines in relation to number of leukocytes, surface area of monolayer formation, and feeding procedures

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Summary

Twenty-eight lymphocytoid cell lines were established within 6 months from the peripheral blood of a single healthy donor. Establishment seemed independent of feeding regimens. Establishment occurred if cultures in 100-ml glass bottles (surface area of 46 cm2) were initiated with a leukocyte population between 4.9×107 and 6.8×107 cells, and if cultures in 16-ml test tubes (surface area of 1.8 cm2) were initiated with a total leukocyte population between 4.9×106 and 2.2×107 cells. It is concluded that establishment is related to a optimal relationship between number of leukocytes and the surface area for possible monolayer formation. When the surface area is decreased, the initial number of leukocytes must be reduced. Establishment of a cell line did not occur with an initial total population below 4.9×106 leukocytes. There is a positive correlation between initial leukocyte number, initial growth velocity, and the determination of establishment by subculture.

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Authors and Affiliations

  1. Roswell Park Memorial Institute, New York State Department of Health, 14203, Buffalo, New York

    Hans W. von Heyden & Dorit von Heyden

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  1. Hans W. von Heyden
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  2. Dorit von Heyden
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This work was supported in part with funds from the Mary B. and L. H. Marshall Foundation.

Hans W. von Heyden is supported by a Fellowship from the Deutsche Forschungsgemeinschaft. After September 1, 1972, the authors will be at the Medizinische Universität Klinik II, Tübingen, West Germany.

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von Heyden, H.W., von Heyden, D. Establishment of lymphocytoid cell lines in relation to number of leukocytes, surface area of monolayer formation, and feeding procedures. In Vitro 8, 309–315 (1973). https://doi.org/10.1007/BF02615912

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  • DOI: https://doi.org/10.1007/BF02615912

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