Summary
Primary monolayer cell cultures of trypsin-dispersed adult rat adenohypophyses are capable of synthetizing deoxyribonucleic acid and are able to divide at least for 3 weeks. Most cells contain secretory granules in the absence of hypothalamic extracts. It seems possible that granules are formed which mature and are released in vitro.
Similar content being viewed by others
References
Stark, E., A. Gyévai, K. Szalay, and Z. Ács. 1965. Hypophyseal-adrenal activity in combined human foetal tissue cultures. Can. J. Phys. Pharmacol. 43: 1–7.
Gaillard, P. J., and A. Schaberg. 1965. Endocrine glands. In: E. N. Willmer (Ed.),Cells and Tissues in Culture. Methods, Biology, and Physiology. Vol. 2. Academic Press, New York, pp. 631–695.
Gailani, S. D., A. Nussbaum, W. J. McDougall, and W. F. McLimans. 1970. Studies on hormone production by human fetal pituitary cell cultures. Proc. Soc. Exp. Biol. Med. 134: 27–32.
Tixier-Vidal, A., and D. Gourdji. 1965. Evolution cytogenique ultrastructurale de l'hypophyse du canard en culture organotypique. Elaboration autonome de prolactine par les explants. C. R. Acad. Sci. (Paris) 261: 805–808.
Tixier-Vidal, A., and D. Gourdji. 1970. Synthesis and renewal of proteins in duck anterior hypophysis in organ culture. J. Cell Biol. 46: 130–136.
Sato, G., G. Augusti-Tocco, M. Posner, and P. Kelly. 1970. Hormone-secreting and hormone responsive cell cultures. In: E. B. Astwood (Ed.),Recent Progress in Hormone Research. Vol. 26. Academic Press, New York, pp. 539–546.
Tashjian, A. H., Jr., F. C. Bancroft, and L. Levine. 1970. Production of both prolactin and growth hormone by clonal strains of rat pituitary tumor cells. Differential effects of hydrocortisone and tissue extracts. J. Cell Biol. 47: 61–70.
Bancroft, F. C., and A. H. Tashjian, Jr. 1971. Growth in suspension culture of rat pituitary cells which produce growth hormone and prolactin. Exp. Cell Res. 64: 125–128.
Ohtsuka, Y., H. Ishikawa, T. Omoto, Y. Takasaki, and F. Yoshimura. 1971. Effect of CRF on the morphological and functional differentiation of the cultured chromophobes isolated from rat anterior pituitaries. Endocrinol. Jap. 18: 133–153.
Pelletier, G., and M. B. Bornstein. 1972. Effect of colchicine on rat anterior pituitary gland in tissue culture. Exp. Cell Res. 70: 221–223.
Tixier-Vidal, A. 1972. Ultrastructural features of gonadotropic pituitary cells in culture. Symp. Biol. Hung., in press.
Farquhar, M. G. 1969. Lysosome function in regulating secretion: disposal of secretory granules in cells of the anterior pituitary gland. In: J. T. Dingle, and H. B. Fell (Eds.),Lysosomes in Biology and Pathology. Vol. 2. North-Holland, Amsterdam, pp. 462–482.
Fawcett, D. W., J. A. Long, and A. L. Jones. 1969. The ultrastructure of endocrine glands. In: E. B. Astwood (Ed.),Recent Progress in in Hormone Research. Vol. 25. Academic Press, New York, pp. 315–380.
Stark, E., A. Gyévai, K. Szalay, and Z. Pósalaky. 1965. Secretion of adrenocorticotrophic hormone by hypophysial cells grown in monolayer culture. J. Endocrinol. 31: 291–292.
Reynolds, E. S. 1963. The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell Biol. 17: 208–212.
Goss, R. J. 1967. The strategy of growth. In: H. Teir, and T. Rytömaa (Eds.),Control of Cellular Growth in Adult Organisms. A. Sigrid Jusélius Foundation Symposium. Academic Press, New York, pp. 3–27.
Crane, W. A., L. P. Dutta, and D. J. Ingle. 1965. Cell proliferation in the rat pituitary. Proc. Soc. Exp. Biol. Med. 119: 167–169.
Nakai, T., T. Watanabe, T. Kigawa, K. Ajika, Y. Murasawa, T. Kobayashi, and T. Kobayashi. 1969. Developmental cell dynamics in the hypothalamus, the anterior pituitary and the ovary of the rat. Endocrinol. Jap. 16: 379–382.
Cameron, I. L. 1970. Cell renewal in the organs and tissues of the nongrowing adult mouse. Tex. Rep. Biol. Med. 28: 203–248.
Mazzei, D., C. Novi, and C. Bazzi. 1966. Mitogenic action of trypsin and chymotrypsin. Lancet ii: 232.
Burger, M. M. 1970. Proteolytic enzymes initiating cell division and escape from contact inhibition of growth. Nature (London) 227: 170–171.
Baserga, R., G. Rovera, and J. Farber. 1971. Control of cellular proliferation in human diploid fibroblasts. In Vitro 7: 80–87.
Koyabashi, T., T. Kigawa, M. Mizuno, and T. Watanabe. 1971. In vitro methods for the study of the adenohypophysial functions to secrete gonadotropin. Acta Endocrinol. Suppl. 153: 27–40.
Guillemin, R., and B. Rosenberg. 1955. Humoral hypothalamic control of anterior pituitary: a study with combined tissue cultures. Endocrinology 57: 599–607.
Guillemin, R., W. R. Hearn, W. R. Cheek, and D. E. Householder. 1957. Control of corticotrophin release: further studies with in vitro methods. Endocrinology 60: 488–506.
Kobayashi, T., T. Kobayashi, T. Kigawa, M. Mizuno, and Y. Amenomori. 1963. Influence of rat hypothalamic extract on gonadotropic activity of cultivated anterior pituitary cells. Endocrinol. Japon. 10: 16–24.
Mittler, J. C., A. Arimura, and A. V. Schally. 1970. Release and synthesis of luteinizing hormone and follicle-stimulating hormone in pituitary cultures in response to hypothalamic preparations. Proc. Soc. Exp. Biol. Med. 133: 1321–1325.
Fleischer, N., and W. E. Rawls. 1970. ACTH synthesis and release in pituitary monolayer culture: effect of dexamethasone. Am. J. Physiol. 219: 445–448.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rappay, G., Gyévai, A., Kondics, L. et al. Growth and fine structure of monolayers derived from adult rat adenohypophyseal cell suspensions. In Vitro 8, 301–306 (1973). https://doi.org/10.1007/BF02615910
Issue Date:
DOI: https://doi.org/10.1007/BF02615910