Skip to main content
SpringerLink
Log in

Human testicular cultures II. Sertoli cells

  • Published:
In Vitro Aims and scope Submit manuscript

Summary

Two cell types, one epithelioid and the other fibroblast-like, were found in human testicular cultures derived from testes of patients. Ultrastructural studies indicated that, whereas the epithelioid cells were Sertoli cells, the fibroblast-like cells were fibroblasts. The Sertoli cells could maintain growth for a period of more than 4 months. In cultures derived from normal testes, only fibroblasts were observed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Leblond, C. P., and Y. Clermont. 1952. Definition of the stages of the cycle of the seminiferous epithelium in the rat. Ann. N. Y. Acad. Sci. 55: 548–570.

    Article  PubMed  CAS  Google Scholar 

  2. Chen, A. T. L., J. A. Reidy, Y. S. Fu, and W. W. Koontz. 1974. Human testicular cultures. I. Chromosome analyses. Proc. Soc. Exp. Biol. Med. 146: 442–447.

    PubMed  CAS  Google Scholar 

  3. Kodani, M., and K. Kondani. 1966. The in vitro cultivation of mammalian Sertoli cells. Proc. Natl. Acad. Sci. U.S.A. 56: 1200–1206.

    Article  PubMed  CAS  Google Scholar 

  4. Jordan, R. T., S. Katsh, and N. Stackelburg. 1961. Spermatocytogenesis with possible spermiogenesis of guinea pig testicular cells grownin vitro. Nature 192: 1053–1055.

    Article  PubMed  CAS  Google Scholar 

  5. Ellingson, D. J., and K. T. S. Yao. 1970. Growth and observations of Chinese hamster seminiferous epitheliumin vitro. J. Cell Sci. 6: 195–205.

    PubMed  CAS  Google Scholar 

  6. Steinberger, A., and E. Steinberger. 1966.In vitro culture of rat testicular cells. Exp. Cell Res. 44: 443–452.

    Article  PubMed  CAS  Google Scholar 

  7. Ferris, R. D., and W. Plowright. 1958. Simplified method for the production of monolayers of testis cells from domestic animals and for the serial examination of monolayer cultures. J. Pathol. Bacteriol. 75: 313–318.

    Article  PubMed  CAS  Google Scholar 

  8. Hess, W. R., H. J. May, and R. E. Patty. 1963. Serial cultures of lamb testicular cells and their use in virus studies. Am. J. Vet. Res. 24: 59–64.

    PubMed  CAS  Google Scholar 

  9. Steinberger, A., and E. Steinberger. 1970. Tissue culture of male mammalian gonads. In Vitro 5: 17–27.

    PubMed  CAS  Google Scholar 

  10. Vilar, O., A. Steinberger, and E. Steinberger. 1967. An electron microscopic study of cultured rat testicular fragments. Z. Zellforsch. Mikrosk. Anat. 78: 221–233.

    Article  PubMed  CAS  Google Scholar 

  11. Vilar, O., A. Steinberger, and E. Steinberger. 1966. Electron microscopy of isolated rat testicular cells grown in vitro. Z. Zellforsch. Mikrosk. Anat. 74: 529–538.

    Article  PubMed  CAS  Google Scholar 

  12. Bawa, S. R. 1963. Fine structure of the Sertoli cell of the human testis. J. Ultrastruct. Res. 9: 459–474.

    Article  Google Scholar 

  13. Brokelmann, J. 1963. Fine structure of germ cells and Sertoli cells during the cycle of the seminiferous epithelium in the rat. Z. Zellforsch. Mikrosk. Anat. 59: 820–850.

    Article  PubMed  CAS  Google Scholar 

  14. Flickinger, C. J. 1967. The postnatal development of the Sertoli cells of the mouse. Z. Zellforsch. Mikrosk. Anat. 78: 92–131.

    Article  PubMed  CAS  Google Scholar 

  15. Lacy, D., and A. J. Pettitt. 1969. Transmission electron microscopy and the production of steroids by the Leydig and Sertoli cells of the human testis. Micron 1: 15–33.

    Google Scholar 

  16. Dym, M. 1974. The fine structure of monkey Sertoli cells in the transitional zone at the junction of the seminiferous tubules with the tubuli recti. Am. J. Anat. 140: 1–26.

    Article  PubMed  CAS  Google Scholar 

  17. Fawcett, D. W., and M. H. Burgos. 1960. Studies on the fine structure of the mammalian testis. II. The human interstitial tissue. Am. J. Anat. 107: 245–269.

    Article  Google Scholar 

  18. de Kretser, D. M. 1967. The fine structure of the testicular interstitial cells in men of normal androgenic status. Z. Zellforsch. Mikrosk. Anat. 80: 594–609.

    Article  PubMed  Google Scholar 

  19. Sohval, A. R., J. L. Gabrilove, and J. Churg. 1973. Ultrastructure of Leydig cell paracrys-talline inclusions, possibly related to Reinke crystals, in the normal human testis. Z. Zellforsch. Mikrosk. Anat. 142: 13–26.

    Article  PubMed  CAS  Google Scholar 

  20. Blackburn, W. R., K. W. Chung, L. Bullock, and C. W. Bardin. 1973. Testicular feminization in the mouse: studies of Leydig cell structure and function. Biol. Reprod. 9: 9–23.

    PubMed  CAS  Google Scholar 

  21. Kay, S., Y. S. Fu, W. W. Koontz, and A. T. L. Chen. 1975. Interstitial cell tumor of the testis. Tissue culture and ultrastructural studies. Am. J. Clin. Pathol. 63: 366–376.

    PubMed  CAS  Google Scholar 

  22. Bloom, W., and D. W. Fawcett. 1968.A Textbook of Histology. 9th ed. W. B. Saunders Co., Philadelphia.

    Google Scholar 

  23. Clermont, Y., and B. Perey. 1957. Quantitative study of the cell population of the seminiferous tubules in immature rats. Am. J. Anat. 100: 241–267.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Andrew T. L. Chen

    Present address: Cellular Genetics Laboratory, Pathology Division, Bureau of Laboratories, Center for Disease Control, 30333, Atlanta, Ga.

Authors and Affiliations

  1. Medical College of Virginia, Virginia Commonwealth University, 23298, Richmond, Virginia

    Andrew T. L. Chen, Yao-Shi Fu & John A. Reidy

Authors
  1. Andrew T. L. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yao-Shi Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. John A. Reidy
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

The study was supported in part by the Institutional Research Funds of Virginia Commonwealth University, and we appreciate the financial support of the Virginia Developmental Disabilities Planning and Advisory Council. We appreciate Dr. James German's critical reading of the manuscript.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, A.T.L., Fu, YS. & Reidy, J.A. Human testicular cultures II. Sertoli cells. In Vitro 11, 313–321 (1975). https://doi.org/10.1007/BF02615642

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02615642

Keywords

Search

Navigation